Physical characterization of a stomatitis papulosa virus genome: a cleavage map for the restriction endonucleases HindIII and EcoRI.

Détails

ID Serval
serval:BIB_98BD7EFC74CB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Physical characterization of a stomatitis papulosa virus genome: a cleavage map for the restriction endonucleases HindIII and EcoRI.
Périodique
Archives of Virology
Auteur(s)
Menna A., Wittek R., Bachmann P.A., Mayr A., Wyler R.
ISSN
0304-8608[print], 0304-8608[linking]
Statut éditorial
Publié
Date de publication
1979
Volume
59
Numéro
1-2
Pages
145-156
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
The genome of stomatitis papulosa virus (a parapoxvirus) was cleaved with the restriction endonucleases HindIII and EcoRI, each giving rise to 6 fragments respectively. Double digestion with both enzymes resulted in 8 bands, two of which contained DNA fragments in double molar concentrations as revealed by reciprocal digests of isolated DNA fragments. The genome size, estimated by summation of the molecular weights of the fragments, is approximately 86 X 10(6) daltons, some 30 X 10(6) daltons smaller than vaccinia virus (an orthopoxvirus) DNA. The cleavage sites of HindIII and EcoRI endonucleases were mapped on the genome by analysis of reciprocal digests of isolated DNA fragments and by cross-hybridization experiments. This yielded two mapped segments which were then oriented relative to one another by cleavage of isolated partial digestion products. The terminal restriction fragments show rapid renaturation after alkali denaturation and subsequent neutralization, indicating that stomatitis papulosa virus DNA contains terminal cross-links analogous to those found in vaccinia virus DNA.
Mots-clé
Animals, Cattle, Cattle Diseases/microbiology, DNA Restriction Enzymes/metabolism, DNA, Viral/analysis, Linkage (Genetics), Molecular Weight, Nucleic Acid Hybridization, Poxviridae/analysis, Poxviridae Infections/veterinary, Pseudocowpox virus/analysis
Pubmed
Web of science
Création de la notice
24/01/2008 11:43
Dernière modification de la notice
20/08/2019 16:00
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