DNA fingerprinting of pearls to determine their origins.

Détails

ID Serval
serval:BIB_982CBDB825E2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
DNA fingerprinting of pearls to determine their origins.
Périodique
PloS one
Auteur(s)
Meyer J.B., Cartier L.E., Pinto-Figueroa E.A., Krzemnicki M.S., Hänni H.A., McDonald B.A.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
8
Numéro
10
Pages
e75606
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
We report the first successful extraction of oyster DNA from a pearl and use it to identify the source oyster species for the three major pearl-producing oyster species Pinctada margaritifera, P. maxima and P. radiata. Both mitochondrial and nuclear gene fragments could be PCR-amplified and sequenced. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay in the internal transcribed spacer (ITS) region was developed and used to identify 18 pearls of unknown origin. A micro-drilling technique was developed to obtain small amounts of DNA while maintaining the commercial value of the pearls. This DNA fingerprinting method could be used to document the source of historic pearls and will provide more transparency for traders and consumers within the pearl industry.

Mots-clé
Animals, DNA Fingerprinting, Pinctada/classification, Pinctada/genetics, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/08/2017 11:00
Dernière modification de la notice
20/08/2019 15:59
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