Hydrogel-based milliwell arrays for standardized and scalable retinal organoid cultures.

Détails

ID Serval
serval:BIB_981417E59C16
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Hydrogel-based milliwell arrays for standardized and scalable retinal organoid cultures.
Périodique
Scientific reports
Auteur(s)
Decembrini S., Hoehnel S., Brandenberg N., Arsenijevic Y., Lutolf M.P.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
24/06/2020
Peer-reviewed
Oui
Volume
10
Numéro
1
Pages
10275
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
The development of improved methods to culture retinal organoids is relevant for the investigation of mechanisms of retinal development under pathophysiological conditions, for screening of neuroprotective compounds, and for providing a cellular source for clinical transplantation. We report a tissue-engineering approach to accelerate and standardize the production of retinal organoids by culturing mouse embryonic stem cells (mESC) in optimal physico-chemical microenvironments. Arrayed round-bottom milliwells composed of biomimetic hydrogels, combined with an optimized medium formulation, promoted the rapid generation of retina-like tissue from mESC aggregates in a highly efficient and stereotypical manner: ∼93% of the aggregates contained retinal organoid structures. 26 day-old retinal organoids were composed of ∼80% of photoreceptors, of which ∼22% are GNAT2-positive cones, an important and rare sensory cell type that is difficult to study in rodent models. The compartmentalization of retinal organoids into predefined locations on a two-dimensional array not only allowed us to derive almost all aggregates into retinal organoids, but also to reliably capture the dynamics of individual organoids, an advantageous requirement for high-throughput experimentation. Our improved retinal organoid culture system should be useful for applications that require scalability and single-organoid traceability.
Pubmed
Open Access
Oui
Création de la notice
03/07/2020 17:28
Dernière modification de la notice
16/07/2020 8:43
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