Extracellular cultivation and morphological characterization of amastigote-like forms of Leishmania panamensis and L. braziliensis

Détails

ID Serval
serval:BIB_96E876475F63
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Extracellular cultivation and morphological characterization of amastigote-like forms of Leishmania panamensis and L. braziliensis
Périodique
Journal of Protozoology
Auteur⸱e⸱s
Eperon  S., McMahon-Pratt  D.
ISSN
0022-3921
Statut éditorial
Publié
Date de publication
10/1989
Volume
36
Numéro
5
Pages
502-10
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep-Oct
Résumé
Two strains of the Leishmania braziliensis complex have been adapted to grow extracellularly at elevated temperature as amastigote-like forms in a cell-free medium. These parasites can be serially cultivated and maintained at 32 degrees C for L. panamensis (WR442; L. braziliensis panamensis) and at 28 degrees C for L. braziliensis (M5052; L. braziliensis braziliensis). Several observations are presented that the forms adapted at elevated temperature are amastigote-like. Morphologically, the amastigote-like organisms appear rounded to ovoid and are immotile and smaller than promastigotes; the flagellum of the amastigote-like forms does not extend beyond the flagellar pocket. In comparison, the promastigotes are very elongated, with a nucleus at mid-cell length and a very long flagellum. By electron microscopy, the short flagellum of the amastigote-like form is within a distended flagellar pocket; the 9 + 2 axonemal configuration is present but the paraxial rod is not observed. By contrast, the flagellum of the promastigote has a paraxial rod which extends from the axosome level. In addition, these amastigote-like forms of Leishmania are able to infect, to survive and to divide within the macrophage cell line J774.
Mots-clé
Animals Cell Line Culture Media Leishmania/*growth & development Leishmania braziliensis/*growth & development/ultrastructure Macrophages/*parasitology Microscopy, Electron Temperature
Pubmed
Web of science
Création de la notice
17/01/2008 13:27
Dernière modification de la notice
20/08/2019 14:58
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