A novel insertion element from Mycobacterium avium, IS1245, is a specific target for analysis of strain relatedness

Détails

ID Serval
serval:BIB_96D631272690
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A novel insertion element from Mycobacterium avium, IS1245, is a specific target for analysis of strain relatedness
Périodique
Journal of Clinical Microbiology
Auteur⸱e⸱s
Guerrero  C., Bernasconi  C., Burki  D., Bodmer  T., Telenti  A.
ISSN
0095-1137 (Print)
Statut éditorial
Publié
Date de publication
02/1995
Volume
33
Numéro
2
Pages
304-7
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
The insertion sequence IS1245 is a novel mycobacterial repetitive element identified in Mycobacterium avium. It encodes a transposase which exhibits a 64% amino acid similarity with IS1081, an insertion element present in the M. tuberculosis complex. The host range of IS1245 appears limited to M. avium as this element was not identified in M. intracellulare or in any other of 18 mycobacteria species tested. When IS1245 was used for restriction fragment length polymorphism (RFLP) analysis, human isolates characteristically presented a high number of copies (median, 16; range, 3 to 27) and a diversity of RFLP patterns comparable to that found by pulsed-field gel electrophoresis. Isolates from nonhuman sources differed both in number of copies and in RFLP pattern diversity: while swine isolates shared the characteristics of human strains, those from several avian sources exhibited a very low copy number of IS1245 and appeared clonal on the basis of RFLP.
Mots-clé
Animals Base Sequence DNA Primers/genetics *DNA Transposable Elements DNA, Bacterial/genetics Gene Amplification Humans Molecular Sequence Data Mycobacterium/genetics Mycobacterium avium/classification/*genetics/isolation & purification Mycobacterium avium Complex/classification/*genetics/isolation & purification Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid Species Specificity
Pubmed
Web of science
Création de la notice
25/01/2008 15:46
Dernière modification de la notice
20/08/2019 15:58
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