Persistence of the same viral strain in early and late relapses of Epstein-Barr virus-associated Hodgkin's disease

Détails

ID Serval
serval:BIB_96575D443B94
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Etude de cas (case report): rapporte une observation et la commente brièvement.
Collection
Publications
Institution
Titre
Persistence of the same viral strain in early and late relapses of Epstein-Barr virus-associated Hodgkin's disease
Périodique
Blood
Auteur(s)
Brousset  P., Schlaifer  D., Meggetto  F., Bachmann  E., Rothenberger  S., Pris  J., Delsol  G., Knecht  H.
ISSN
0006-4971 (Print)
Statut éditorial
Publié
Date de publication
10/1994
Volume
84
Numéro
8
Pages
2447-51
Notes
Case Reports
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Résumé
Twelve cases of relapsing Hodgkin's disease were investigated for the presence of Epstein-Barr virus (EBV). Of these, 7 cases contained EBV gene products (LMP1, EBER RNA) in the diagnostic Reed-Sternberg cells and variants at first presentation and at relapse(s), whereas 5 cases were negative at both first diagnosis and relapse. Among the 7 EBV-positive cases, material for DNA extraction was available in 2 cases at both diagnosis and relapse(s). Ig and T-cell receptor gene rearrangements displayed a germline configuration in the 2 cases. However, Southern blot analysis of the terminal repeats (TR) of EBV genome showed that, in 1 of the 2 cases, the fragment was of the same size at diagnosis and in the subsequent two relapses (1 early and 1 late). The second case contained monoclonal EBV genome at diagnosis, but the Southern analysis of the TR was negative at relapse. The latent membrane protein (LMP1) sequence analysis confirmed the persistence of a distinctive viral strain in each of the 2 cases with individual abnormalities within the carboxy terminal region (5 point mutations and a 30-bp deletion for the first case and 6 point mutations for the second case). The persistence of a given strain in early and late relapses is evidence towards the view that in Hodgkin's disease such relapses are related to a single residual tumor cell clone.
Mots-clé
Adult Antigens, Viral/genetics Blotting, Southern DNA, Viral/analysis Gene Rearrangement Gene Rearrangement, T-Lymphocyte *Herpesvirus 4, Human/*genetics/isolation & purification Hodgkin Disease/pathology/*virology Humans Immunohistochemistry In Situ Hybridization Male Middle Aged *Neoplasm Recurrence, Local Polymorphism, Genetic RNA-Binding Proteins/genetics Reed-Sternberg Cells/virology Repetitive Sequences, Nucleic Acid *Ribosomal Proteins Viral Matrix Proteins/genetics
Pubmed
Web of science
Création de la notice
25/01/2008 14:36
Dernière modification de la notice
20/08/2019 14:58
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