The murine orthologue of the Golgi-localized TPTE protein provides clues to the evolutionary history of the human TPTE gene family.

Détails

ID Serval
serval:BIB_964F6533BD0C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The murine orthologue of the Golgi-localized TPTE protein provides clues to the evolutionary history of the human TPTE gene family.
Périodique
Human Genetics
Auteur(s)
Guipponi M., Tapparel C., Jousson O., Scamuffa N., Mas C., Rossier C., Hutter P., Meda P., Lyle R., Reymond A., Antonarakis S.E.
ISSN
0340-6717 (Print)
ISSN-L
0340-6717
Statut éditorial
Publié
Date de publication
2001
Volume
109
Numéro
6
Pages
569-575
Langue
anglais
Résumé
The human TPTE gene encodes a testis-specific protein that contains four potential transmembrane domains and a protein tyrosine phosphatase motif, and shows homology to the tumor suppressor PTEN/MMAC1. Chromosomal mapping revealed multiple copies of the TPTE gene present on the acrocentric chromosomes 13, 15, 21 and 22, and the Y chromosome. Zooblot analysis suggests that mice may possess only one copy of TPTE. In the present study, we report the isolation and initial characterization of the full-length cDNA of the mouse homologue Tpte. At least three different mRNA transcripts ( Tpte.a, b, c) are produced via alternative splicing, encoding predicted proteins that would contain four potential transmembrane domains and a protein tyrosine phosphatase motif. Transfection of a 5'EGFP-TPTE fusion protein in Hela cells revealed an intracellular localization within the Golgi apparatus. Tpte was mapped by radiation hybrid to a region of mouse chromosome 8 that shows conserved synteny with human 13q14.2-q21 between NEK3 and SGT1. This region of the human genome was found to contain a partial, highly diverged copy of TPTE that is likely to represent the ancestral copy from which the other copies of TPTE arose through duplication events. The Y chromosome copy of TPTE is a pseudogene and is not therefore involved in the testis expression of this gene family.
Mots-clé
Amino Acid Motifs, Amino Acid Sequence, Animals, Cell Compartmentation, Chromosome Mapping, Chromosomes, Human, Pair 13, Evolution, Molecular, Golgi Apparatus/chemistry, Humans, Male, Membrane Proteins/genetics, Mice, Molecular Sequence Data, Multigene Family/genetics, PTEN Phosphohydrolase, Phosphoric Monoester Hydrolases/genetics, Phylogeny, Protein Tyrosine Phosphatases, Sequence Homology, Amino Acid, Tumor Suppressor Proteins/genetics, Vertebrates/classification, Vertebrates/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 15:52
Dernière modification de la notice
20/08/2019 14:58
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