Biophysical properties and morphology of purified antigen associated with non-A, non-B hepatitis.

Détails

ID Serval
serval:BIB_964BA962A3B9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Biophysical properties and morphology of purified antigen associated with non-A, non-B hepatitis.
Périodique
Medical Microbiology and Immunology
Auteur⸱e⸱s
Spertini O., Frei P.C.
ISSN
0300-8584
Statut éditorial
Publié
Date de publication
1986
Peer-reviewed
Oui
Volume
175
Numéro
4
Pages
229-239
Langue
anglais
Notes
Publication types: Journal Article
Résumé
The antigen from a non-A, non-B antigen-antibody system previously described was purified and, when tested by immunodiffusion, was shown to produce patterns of identity with all antigen-positive sera of the system. The sedimentation coefficient S20,w of the antigen was estimated by rate zonal ultracentrifugation to be 48. Isopycnic ultracentrifugation in CsCl gradient of non-A, non-B antigen generated a single sedimentation peak at the density of 1.28. SDS-polyacrylamide-agarose gel electrophoresis showed that I125-labelled antigen migrated as a single band (molecular weight 3.0 X 10(6)); in addition, labelled material also migrated to the albumin region. With SDS-PAGE under reducing conditions, the antigen was shown to consist of seven polypeptides. Spherical particles of 23 nm in diameter, demonstrated by electron microscopy, could be aggregated by purified non-A, non-B antibody. They probably represent the antigen and not a complete viral particle. The characteristics of this antigen associated with non-A, non-B hepatitis appear therefore to be close to those of hepatitis B surface antigen.
Mots-clé
Antigens, Viral/analysis, Autoradiography, Centrifugation, Isopycnic, Electrophoresis, Polyacrylamide Gel, Hepatitis C/immunology, Hepatitis C Antigens, Hepatitis, Viral, Human/immunology, Humans, Immunodiffusion, Microscopy, Electron, Molecular Weight
Pubmed
Web of science
Création de la notice
25/01/2008 15:31
Dernière modification de la notice
20/08/2019 14:58
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