Determination of the polyfructosan sinistrin in biological fluids by HPLC with electrochemical detection

Détails

ID Serval
serval:BIB_95D69C8B2A75
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Determination of the polyfructosan sinistrin in biological fluids by HPLC with electrochemical detection
Périodique
Journal of Pharmaceutical and Biomedical Analysis
Auteur⸱e⸱s
Sechaud  R., Decosterd  L. A., Pechere-Bertschi  A., Biollaz  J., Kesselring  U. W.
ISSN
0731-7085 (Print)
Statut éditorial
Publié
Date de publication
02/1996
Volume
14
Numéro
4
Pages
483-90
Notes
Journal Article --- Old month value: Feb
Résumé
A sensitive HPLC method with electrochemical detection was developed for the determination of the polyfructosan sinistrin in human plasma and urine. Proteins and interfering components such as glucose were removed from plasma and urine samples by solid phase extraction on C18 cartridges. Chromatographic separations were achieved at 85 degrees C on a 300 mm x 7.8 mm i.d. column, using ion moderated partition chromatography with distilled water at a flow rate of 0.6 ml min-1. After post-column addition of NaOH 0.3 M (0.6 ml min-1), the electrochemical detection of the eluate was performed with a sequence of three potentials (0.05 V, -0.8 V, 0.6 V) of specific pulse duration 300, 100 and 100 ms respectively. Xylose was used as internal standard for the quantitative determinations. The calibration curves were linear (r2 > 0.992) over the working range 5-300 micrograms ml-1. This method has been characterized, validated and applied successfully in a study comparing two modes of glomerular filtration rate determination in healthy volunteers (bolus vs. constant rate infusion of sinistrin).
Mots-clé
Chromatography, High Pressure Liquid/methods Electrochemistry Glomerular Filtration Rate Humans Oligosaccharides/*blood/pharmacokinetics/*urine
Pubmed
Web of science
Création de la notice
25/01/2008 10:41
Dernière modification de la notice
20/08/2019 14:58
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