Identification of a novel member of the CLIC family, CLIC6, mapping to 21q22.12.

Détails

ID Serval
serval:BIB_95C6854D9632
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Identification of a novel member of the CLIC family, CLIC6, mapping to 21q22.12.
Périodique
Gene
Auteur⸱e⸱s
Friedli M., Guipponi M., Bertrand S., Bertrand D., Neerman-Arbez M., Scott H.S., Antonarakis S.E., Reymond A.
ISSN
0378-1119 (Print)
ISSN-L
0378-1119
Statut éditorial
Publié
Date de publication
2003
Volume
320
Pages
31-40
Langue
anglais
Résumé
Five members of the newly identified chloride intracellular channel (CLIC) gene family of intracellular chloride channels (CLIC1-CLIC5) have previously been described in humans. Here we report the molecular cloning and initial characterisation of two splice forms of a novel member of this family, CLIC6, mapping to human chromosome 21. Two essential features distinguish CLIC6 from other members of the family. The CLIC6 protein is significantly longer and the CLIC6 gene contains a GC rich segment, which encodes a 10 amino acid motif repeated 14 times in the amino-terminus. Surprisingly, the repeat is conserved in the lagomorphs, but not in the rodents lineage. The putative bovine orthologue of CLIC5, p64, also exhibits a repeated motif, which is different from that of CLIC6. Attempts to functionally characterise CLIC6 by voltage clamp failed to show any chloride channel activity. Hence, the exact function of this protein remains unknown.
Mots-clé
Alternative Splicing, Amino Acid Sequence, Animals, COS Cells, Cell Line, Cercopithecus aethiops, Chloride Channels/genetics, Chromosome Mapping, Chromosomes, Human, Pair 21/genetics, Cloning, Molecular, DNA, Complementary/chemistry, DNA, Complementary/genetics, Female, Gene Expression, Green Fluorescent Proteins, Humans, Luminescent Proteins/genetics, Luminescent Proteins/metabolism, Membrane Potentials/physiology, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Inbred Strains, Microscopy, Fluorescence, Molecular Sequence Data, Oocytes/metabolism, Oocytes/physiology, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Repetitive Sequences, Nucleic Acid/genetics, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transfection, Xenopus
Pubmed
Web of science
Création de la notice
24/01/2008 16:51
Dernière modification de la notice
20/08/2019 15:58
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