Dominant TNF-α+ Mycobacterium tuberculosis-specific CD4+ T cell responses discriminate between latent infection and active disease.

Détails

ID Serval
serval:BIB_9537273936CE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Dominant TNF-α+ Mycobacterium tuberculosis-specific CD4+ T cell responses discriminate between latent infection and active disease.
Périodique
Nature medicine
Auteur(s)
Harari A., Rozot V., Bellutti Enders F., Perreau M., Mazza -Stalder J.M., Nicod L.P., Cavassini M., Calandra T., Blanchet C.L., Jaton K., Faouzi M., Day C.L., Hanekom W.A., Bart P.A., Pantaleo G.
ISSN
1546-170X (Electronic)
ISSN-L
1078-8956
Statut éditorial
Publié
Date de publication
03/2011
Peer-reviewed
Oui
Volume
17
Numéro
3
Pages
372-376
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Rapid diagnosis of active Mycobacterium tuberculosis (Mtb) infection remains a clinical and laboratory challenge. We have analyzed the cytokine profile (interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2)) of Mtb-specific T cells by polychromatic flow cytometry. We studied Mtb-specific CD4+ T cell responses in subjects with latent Mtb infection and active tuberculosis disease. The results showed substantial increase in the proportion of single-positive TNF-α Mtb-specific CD4+ T cells in subjects with active disease, and this parameter was the strongest predictor of diagnosis of active disease versus latent infection. We validated the use of this parameter in a cohort of 101 subjects with tuberculosis diagnosis unknown to the investigator. The sensitivity and specificity of the flow cytometry-based assay were 67% and 92%, respectively, the positive predictive value was 80% and the negative predictive value was 92.4%. Therefore, the proportion of single-positive TNF-α Mtb-specific CD4+ T cells is a new tool for the rapid diagnosis of active tuberculosis disease.
Mots-clé
CD4-Positive T-Lymphocytes/immunology, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Humans, Mycobacterium tuberculosis/immunology, Tuberculosis/immunology, Tumor Necrosis Factor-alpha/immunology
Pubmed
Web of science
Création de la notice
28/01/2021 17:08
Dernière modification de la notice
29/01/2021 7:26
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