Differential expression of epithelial sodium channel subunit mRNAs in rat skin

Détails

ID Serval
serval:BIB_951805042989
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differential expression of epithelial sodium channel subunit mRNAs in rat skin
Périodique
Journal of Cell Science
Auteur⸱e⸱s
Roudier-Pujol  C., Rochat  A., Escoubet  B., Eugene  E., Barrandon  Y., Bonvalet  J. P., Farman  N.
ISSN
0021-9533 (Print)
Statut éditorial
Publié
Date de publication
02/1996
Volume
109 ( Pt 2)
Pages
379-85
Notes
Journal Article --- Old month value: Feb
Résumé
Three subunits (alpha, beta, gamma) of the amiloride-sensitive epithelial sodium channel have been recently characterized. The channel subunits have significant homologies with the Caenorhabditis elegans mec-4, mec-10 and deg-1 genes, which are involved in control of cell volume and mecanotransduction. These subunits are coexpressed at equivalent levels in the renal collecting duct and the distal colon epithelium which are high resistance sodium transporting epithelia. We have investigated whether these subunits were expressed, at the mRNA level, in transporting as well as non transporting epithelial cells of rat skin. In full-thickness abdominal skin only alpha and gamma subunit mRNAs were detected, while all three subunit mRNAs were present in sole skin, as demonstrated by RNase-protection assay. Furthermore, the level of expression of each subunit varied with the epithelial cell type as demonstrated by in situ hybridization: epidermal and follicular keratinocytes express mostly alpha and gamma subunits (while beta was low); a prevalence of beta and gamma was observed in sweat glands. Thus, it appeared that two out of the three subunit mRNAs predominated in each epithelial structure. In addition, mRNAs of the alpha, beta and gamma subunits of the amiloride-sensitive sodium channel were expressed at a higher level in large suprabasal epidermal keratinocytes (which undergo terminal differentiation) than in small proliferative basal keratinocytes.
Mots-clé
Animals Epidermis/*metabolism Epithelium Gene Expression Hair Follicle/*metabolism Male RNA, Messenger Rats Rats, Sprague-Dawley Skin/metabolism Sodium Channels/genetics/*metabolism Sweat Glands/*metabolism
Pubmed
Web of science
Création de la notice
28/01/2008 8:41
Dernière modification de la notice
20/08/2019 14:57
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