Optimal activation of tumor-reactive T cells by selected antigenic peptide analogues.

Détails

Ressource 1Télécharger: serval:BIB_943F39FA15D7.P001 (222.37 [Ko])
Etat: Public
Version: de l'auteur
Licence: Non spécifiée
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ID Serval
serval:BIB_943F39FA15D7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Optimal activation of tumor-reactive T cells by selected antigenic peptide analogues.
Périodique
International immunology
Auteur(s)
Valmori D., Fonteneau J.F., Valitutti S., Gervois N., Dunbar R., Liénard D., Rimoldi D., Cerundolo V., Jotereau F., Cerottini J.C., Speiser D.E., Romero P.
ISSN
0953-8178
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
11
Numéro
12
Pages
1971-80
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Many mechanisms have been proposed to explain why immune responses against human tumor antigens are generally ineffective. For example, tumor cells have been shown to develop active immune evasion mechanisms. Another possibility is that tumor antigens are unable to optimally stimulate tumor-specific T cells. In this study we have used HLA-A2/Melan-A peptide tetramers to directly isolate antigen-specific CD8(+) T cells from tumor-infiltrated lymph nodes. This allowed us to quantify the activation requirements of a representative polyclonal yet monospecific tumor-reactive T cell population. The results obtained from quantitative assays of intracellular Ca(2+) mobilization, TCR down-regulation, cytokine production and induction of effector cell differentiation indicate that the naturally produced Melan-A peptides are weak agonists and are clearly suboptimal for T cell activation. In contrast, optimal T cell activation was obtained by stimulation with recently defined peptide analogues. These findings provide a molecular basis for the low immunogenicity of tumor cells and suggest that patient immunization with full agonist peptide analogues may be essential for stimulation and maintenance of anti-tumor T cell responses in vivo.
Mots-clé
Antigens, Neoplasm, Calcium, Cell Line, Cytokines, Cytotoxicity, Immunologic, Humans, Lymphocyte Activation, Melanoma, Neoplasm Proteins, Receptors, Antigen, T-Cell, T-Lymphocytes
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
25/09/2019 7:10
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