Construction of Multifragment Plasmids by Homologous Recombination in Yeast.

Détails

ID Serval
serval:BIB_941960596A88
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Construction of Multifragment Plasmids by Homologous Recombination in Yeast.
Périodique
Cold Spring Harbor protocols
Auteur(s)
van Leeuwen J., Andrews B., Boone C., Tan G.
ISSN
1559-6095 (Electronic)
ISSN-L
1559-6095
Statut éditorial
Publié
Date de publication
01/09/2015
Volume
2015
Numéro
9
Pages
pdb.top084111
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
Over the past decade, the focus of cloning has shifted from constructing plasmids that express a single gene of interest to creating multigenic constructs that contain entire pathways or even whole genomes. Traditional cloning methods that rely on restriction digestion and ligation are limited by the number and size of fragments that can efficiently be combined. Here, we focus on the use of homologous-recombination-based DNA manipulation in the yeast Saccharomyces cerevisiae for the construction of plasmids from multiple DNA fragments. Owing to its simplicity and high efficiency, cloning by homologous recombination in yeast is very accessible and can be applied to high-throughput construction procedures. Its applications extend beyond yeast-centered purposes and include the cloning of large mammalian DNA sequences and entire bacterial genomes.
Mots-clé
Cloning, Molecular/methods, Homologous Recombination, Molecular Biology/methods, Plasmids/isolation & purification, Saccharomyces cerevisiae/genetics
Pubmed
Création de la notice
22/01/2019 16:07
Dernière modification de la notice
21/08/2019 5:35
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