Application and evaluation of double digest selective label (DDSL) typing technique for Pseudomonas aeruginosa hospital isolates.

Détails

ID Serval
serval:BIB_94082899750D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Application and evaluation of double digest selective label (DDSL) typing technique for Pseudomonas aeruginosa hospital isolates.
Périodique
Journal of Microbiological Methods
Auteur(s)
Terletskiy V., Kuhn G., Francioli P., Blanc D.S.
ISSN
0167-7012
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
72
Numéro
3
Pages
283-7
Langue
anglais
Résumé
This study describes the application and evaluation of a recently developed fast bacterial typing technique (double digest selective label - DDSL) for hospital isolates of Pseudomonas aeruginosa. The protocol was based on a simultaneous double digestion/labelling reaction which was performed in a single reaction tube. After agarose gel separation selectively tagged restriction fragments were transferred using deonised water to a nylon membrane and visualized by a colour reaction. Starting from overnight culture, turn around time using this technique was only 8 h. The DDSL typing technique was applied for 77 hospital isolates. Among them 63 isolates were also typed by PFGE and the typing results were compared with those of DDSL. In conclusion, both techniques discriminated bacterial isolates into the same major clusters. DDSL proved to be as discriminatory as PFGE but much faster and easier to set up in a standard microbiological laboratory.
Mots-clé
Bacterial Typing Techniques, Cluster Analysis, Cross Infection, DNA Fingerprinting, DNA, Bacterial, Electrophoresis, Gel, Pulsed-Field, Genotype, Hospitals, Humans, Pseudomonas Infections, Pseudomonas aeruginosa, Time Factors
Pubmed
Web of science
Création de la notice
05/02/2008 13:13
Dernière modification de la notice
20/08/2019 15:56
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