Improvement of mesh recolonization in abdominal wall reconstruction with adipose vs. bone marrow mesenchymal stem cells in a rodent model.

Détails

ID Serval
serval:BIB_939D2E8B8356
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Improvement of mesh recolonization in abdominal wall reconstruction with adipose vs. bone marrow mesenchymal stem cells in a rodent model.
Périodique
Journal of pediatric surgery
Auteur(s)
van Steenberghe M., Schubert T., Guiot Y., Goebbels R.M., Gianello P.
ISSN
1531-5037 (Electronic)
ISSN-L
0022-3468
Statut éditorial
Publié
Date de publication
08/2017
Peer-reviewed
Oui
Volume
52
Numéro
8
Pages
1355-1362
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Reconstruction of muscle defects remains a challenge. Our work assessed the potential of an engineered construct made of a human acellular collagen matrix (HACM) seeded with porcine mesenchymal stem cells (MSCs) to reconstruct abdominal wall muscle defects in a rodent model.
This study compared 2 sources of MSCs (bone-marrow, BMSCs, and adipose, ASCs) in vitro and in vivo for parietal defect reconstruction. Cellular viability and growth factor release (VEGF, FGF-Beta, HGF, IGF-1, TGF-Beta) were investigated under normoxic/hypoxic culture conditions. Processed and recellularized HACMs were mechanically assessed. The construct was tested in vivo in full thickness abdominal wall defect treated with HACM alone vs. HACM+ASCs or BMSCs (n=14). Tissue remodeling was studied at day 30 for neo-angiogenesis and muscular reconstruction.
A significantly lower secretion of IGF was observed with ASCs vs. BMSCs under hypoxic conditions (-97.6%, p<0.005) whereas significantly higher VEGF/FGF secretions were found with ASCs (+92%, p<0.001 and +72%, p<0.05, respectively). Processing and recellularization did not impair the mechanical properties of the HACM. In vivo, angiogenesis and muscle healing were significantly improved by the HACM+ASCs in comparison to BMSCs (p<0.05) at day 30.
A composite graft made of an HACM seeded with ASCs can improve muscle repair by specific growth factor release in hypoxic conditions and by in vivo remodeling (neo-angiogenesis/graft integration) while maintaining mechanical properties.

Mots-clé
Abdominal Wall/surgery, Abdominoplasty/methods, Adipose Tissue/cytology, Adipose Tissue/transplantation, Animals, Bone Marrow Cells/cytology, Cell Differentiation, Cells, Cultured, Collagen/pharmacology, Disease Models, Animal, Hernia, Abdominal/pathology, Hernia, Abdominal/surgery, Humans, Mesenchymal Stromal Cells/cytology, Swine, Adipose mesenchymal stem cells, Angiogenesis, Biological mesh recolonization, Bone marrow mesenchymal stem cells, Human collagen matrix, Tissue remodeling
Pubmed
Web of science
Création de la notice
11/09/2017 18:43
Dernière modification de la notice
20/08/2019 15:56
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