Human natural Treg microRNA signature: role of microRNA-31 and microRNA-21 in FOXP3 expression.

Détails

ID Serval
serval:BIB_92EC3261647A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Human natural Treg microRNA signature: role of microRNA-31 and microRNA-21 in FOXP3 expression.
Périodique
European journal of immunology
Auteur⸱e⸱s
Rouas R., Fayyad-Kazan H., El Zein N., Lewalle P., Rothé F., Simion A., Akl H., Mourtada M., El Rifai M., Burny A., Romero P., Martiat P., Badran B.
ISSN
1521-4141[electronic]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
39
Numéro
6
Pages
1608-1618
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Treg are the main mediators of dominant tolerance. Their mechanisms of action and applications are subjects of considerable debate currently. However, a human microRNA (miR) Treg signature has not been described yet. We investigated human natural Treg and identified a signature composed of five miR (21, 31, 125a, 181c and 374). Among those, two were considerably under-expressed (miR-31 and miR-125a). We identified a functional target sequence for miR-31 in the 3' untranslated region (3' UTR) of FOXP3 mRNA. Using lentiviral transduction of fresh cord blood T cells, we demonstrated that miR-31 and miR-21 had an effect on FOXP3 expression levels. We showed that miR-31 negatively regulates FOXP3 expression by binding directly to its potential target site in the 3' UTR of FOXP3 mRNA. We next demonstrated that miR-21 acted as a positive, though indirect, regulator of FOXP3 expression. Transduction of the remaining three miR had no direct effect on FOXP3 expression or on the phenotype and will remain the subject of future investigations. In conclusion, not only have we identified and validated a miR signature for human natural Treg, but also unveiled some of the mechanisms by which this signature was related to the control of FOXP3 expression in these cells.
Mots-clé
3' Untranslated Regions/genetics, 3' Untranslated Regions/metabolism, CD4-Positive T-Lymphocytes/metabolism, Fetal Blood/cytology, Forkhead Transcription Factors/genetics, Gene Expression/genetics, Gene Expression Profiling, Gene Expression Regulation, Hela Cells, Humans, Lentivirus/genetics, MicroRNAs/antagonists & inhibitors, MicroRNAs/genetics, Oligonucleotides, Antisense/genetics, T-Lymphocyte Subsets/metabolism, T-Lymphocytes, Regulatory/metabolism, Transduction, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/01/2010 14:55
Dernière modification de la notice
20/08/2019 14:55
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