Identification and quantitation of angiotensins

Détails

ID Serval
serval:BIB_92ABE69DEEC9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Identification and quantitation of angiotensins
Périodique
Journal of Cardiovascular Pharmacology
Auteur(s)
Nussberger  J., Buhler  K., Waeber  B., Brunner  H. R.
ISSN
0160-2446 (Print)
Statut éditorial
Publié
Date de publication
1986
Volume
8 Suppl 10
Pages
S23-8
Notes
Journal Article
Résumé
The introduction of pharmacological inhibitors of renin and angiotensin (ANG) converting enzyme in cardiovascular therapy drew new attention to the role of the renin-angiotensin system in regulating circulatory homeostasis. Accurate measurement of very low concentration of the biologically active peptides ANG II and ANG III sometimes in the presence of large amounts of inactive precursors and metabolites is needed. Active and inactive angiotensins have been measured specifically in attomolar quantities by radioimmunoassay after rapid extraction on bonded-phase silica and subsequent high-performance liquid chromatography. ANG II was found to be generated in cold plasma containing conventional inhibitors; it was concluded that renin, converting enzyme, and angiotensinases must be inhibited if physiological ANG concentrations are to be estimated. Modification of the antigen enhanced the specificity of the ANG II antisera. Monoclonal antibodies to ANG II were produced.
Mots-clé
Angiotensin II/biosynthesis/*blood Antibodies, Monoclonal Antigen-Antibody Complex Chromatography, High Pressure Liquid Cross Reactions Humans Radioimmunoassay/methods
Pubmed
Web of science
Création de la notice
05/03/2008 17:39
Dernière modification de la notice
20/08/2019 15:55
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