A negative regulatory pathway of GLUT4 trafficking in adipocyte: new function of RIP140 in the cytoplasm via AS160.

Détails

ID Serval
serval:BIB_92591F2734B4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A negative regulatory pathway of GLUT4 trafficking in adipocyte: new function of RIP140 in the cytoplasm via AS160.
Périodique
Cell metabolism
Auteur(s)
Ho P.C., Lin Y.W., Tsui Y.C., Gupta P., Wei L.N.
ISSN
1932-7420 (Electronic)
ISSN-L
1550-4131
Statut éditorial
Publié
Date de publication
12/2009
Peer-reviewed
Oui
Volume
10
Numéro
6
Pages
516-523
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural
Publication Status: ppublish
Résumé
Receptor-interacting protein 140 (RIP140), a nuclear receptor corepressor, is important for lipid and glucose metabolism. In adipocytes, RIP140 can be phosphorylated by protein kinase C epsilon (PKCvarepsilon), followed by arginine methylation, and exported to the cytoplasm. This study demonstrates for the first time a cytoplasmic function for RIP140: to counteract insulin-stimulated glucose transporter 4 (GLUT4) membrane partitioning and glucose uptake in adipocytes. Cytoplasmic RIP140 interacts with the Akt substrate AS160, thereby impeding AS160 phosphorylation by Akt; this in turn reduces GLUT4 trafficking. This signal transduction pathway can be recapitulated in the epididymal adipocytes of diet-induced obese mice: nuclear PKCvarepsilon is activated, cytoplasmic RIP140 increases, and GLUT4 trafficking and glucose uptake are reduced. The data reveal a new, cytoplasmic function for RIP140 as a negative regulator of GLUT4 trafficking and glucose uptake, and shed insight into the regulation of basal and insulin-stimulated glucose disposal by a nuclear-initiated counteracting mechanism.
Mots-clé
Adaptor Proteins, Signal Transducing/metabolism, Adipocytes/metabolism, Animals, Cell Line, Cytoplasm/metabolism, Down-Regulation, GTPase-Activating Proteins/metabolism, Glucose/metabolism, Glucose Transporter Type 4/metabolism, Mice, Mice, Obese, Nuclear Proteins/metabolism, Nuclear Receptor Interacting Protein 1, Protein Kinase C-epsilon/metabolism, Protein Transport, Proto-Oncogene Proteins c-akt/metabolism, Signal Transduction, Subcutaneous Fat/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
05/04/2019 16:37
Dernière modification de la notice
20/08/2019 15:55
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