Role of Inflammatory Monocytes in Vaccine-Induced Reduction of Helicobacter felis Infection.

Détails

ID Serval
serval:BIB_9253CD28D90D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Role of Inflammatory Monocytes in Vaccine-Induced Reduction of Helicobacter felis Infection.
Périodique
Infection and Immunity
Auteur(s)
Moyat M., Mack M., Bouzourene H., Velin D.
ISSN
1098-5522 (Electronic)
ISSN-L
0019-9567
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
83
Numéro
11
Pages
4217-4228
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
Despite the proven ability of immunization to reduce Helicobacter infection in mouse models, the precise mechanism of protection has remained elusive. In this study, we evaluated the role of inflammatory monocytes in the vaccine-induced reduction of Helicobacter felis infection. We first showed by using flow cytometric analysis that Ly6C(low) major histocompatibility complex class II-positive chemokine receptor type 2 (CCR2)-positive CD64(+) inflammatory monocytes accumulate in the stomach mucosa during the vaccine-induced reduction of H. felis infection. To determine whether inflammatory monocytes played a role in the protection, these cells were depleted with anti-CCR2 depleting antibodies. Indeed, depletion of inflammatory monocytes was associated with an impaired vaccine-induced reduction of H. felis infection on day 5 postinfection. To determine whether inflammatory monocytes had a direct or indirect role, we studied their antimicrobial activities. We observed that inflammatory monocytes produced tumor necrosis factor alpha and inducible nitric oxide synthase (iNOS), two major antimicrobial factors. Lastly, by using a Helicobacter in vitro killing assay, we showed that mouse inflammatory monocytes and activated human monocytes killed H. pylori in an iNOS-dependent manner. Collectively, these data show that inflammatory monocytes play a direct role in the immunization-induced reduction of H. felis infection from the gastric mucosa.
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/11/2015 18:24
Dernière modification de la notice
20/08/2019 15:55
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