Unusual distribution of DNA methylation within the hTERT CpG island in tissues and cell lines

Détails

ID Serval
serval:BIB_91F4FB014C54
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Unusual distribution of DNA methylation within the hTERT CpG island in tissues and cell lines
Périodique
Biochemical and Biophysical Research Communications
Auteur(s)
Guilleret  I., Benhattar  J.
ISSN
0006-291X (Print)
Statut éditorial
Publié
Date de publication
2004
Volume
325
Numéro
3
Pages
1037-1043
Notes
PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't
Résumé
The promoter region of the gene encoding the human telomerase reverse transcriptase (hTERT) is located in a CpG island and was shown to be regulated, at least in part, by DNA methylation. However, the observed correlation between hTERT methylation and gene expression was opposite to the general model of regulation by DNA methylation. We established a detailed mapping of methylcytosines at the CpG island (-1539 to +1732) surrounding the hTERT promoter in tissues and cell lines. In telomerase-positive samples, a methylation of all the CpG sites was observed for the hTERT promoter region (-500 to +1), whereas the exonic part (+1 to +450) revealed an unstable methylation pattern. Incomplete methylation of the proximal exon region could be necessary for, at least, a low level of hTERT transcription. In conclusion, hypermethylation of the CpG island plays a complex but essential role in the expression of hTERT in telomerase-positive cells
Mots-clé
Cell Line,Tumor/CpG Islands/genetics/DNA Methylation/DNA-Binding Proteins/Gene Expression Regulation,Neoplastic/Humans/Neoplasms/Organ Specificity/Promoter Regions (Genetics)/Sequence Analysis,DNA/methods/Sequence Homology,Nucleic Acid/Telomerase
Pubmed
Web of science
Création de la notice
29/01/2008 19:33
Dernière modification de la notice
20/08/2019 15:55
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