Detecting and analyzing murine innate lymphoid cells.

Détails

ID Serval
serval:BIB_91629D320BEA
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Detecting and analyzing murine innate lymphoid cells.
Périodique
Methods in enzymology
Auteur⸱e⸱s
Gomez-Cadena A., Romero P., Trabanelli S., Jandus C.
ISSN
1557-7988 (Electronic)
ISSN-L
0076-6879
Statut éditorial
Publié
Date de publication
2020
Peer-reviewed
Oui
Volume
631
Pages
329-342
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
During the last 10 years, different subsets of Innate lymphoid cells (ILCs) have been identified in murine models. ILCs play and important role in maintaining immune barriers, tissue homeostasis, and are able to regulate the immune response in several anatomic sites. They can be found in lymphoid and non-lymphoid organs of adult mice but are mainly tissue-resident cells that can expand locally under physiologic or pathologic conditions (Gasteiger, Fan, Dikiy, Lee, & Rudensky, 2015). Because ILCs need to be identified by a complex combination of several cell-surface and intracellular markers and by their production of specific sets of cytokines, multiparametric flow cytometry remains one of the most efficient methods to analyze and isolate the different ILC sub-populations. This chapter describes how ILCs can be identified in different murine organs and how ILC subsets can be isolated and functionally analyzed.
Mots-clé
Animals, Flow Cytometry/methods, Immunity, Innate, Immunologic Techniques, Lymphocytes/classification, Lymphocytes/immunology, Lymphocytes/metabolism, Mice, Immune regulation, Innate immunity, Innate lymphoid cells (ILC)
Pubmed
Web of science
Création de la notice
23/01/2020 16:43
Dernière modification de la notice
25/02/2021 7:26
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