Detection of extended-spectrum ss-lactamase-producing Enterobacteriaceae in rectal swabs with the Mastdiscs ID AmpC ssLSE detection set

Détails

ID Serval
serval:BIB_908E76E19846
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Detection of extended-spectrum ss-lactamase-producing Enterobacteriaceae in rectal swabs with the Mastdiscs ID AmpC ssLSE detection set
Périodique
Pathol Biol (Paris)
Auteur(s)
Lemaitre N., Loiez C., Pastourel N., Grandbastien B., Loukili N., Dessein R., Courcol R.
ISSN
0369-8114
Statut éditorial
Publié
Date de publication
10/2012
Volume
60
Numéro
5
Pages
e41–4
Langue
français
Résumé
OBJECTIVES: The double-disk synergy test was compared to the Mastdiscs ID AmpC and ESssL method for detection of ESssL production in rectal swab. METHODS: Two hundred and forty-nine rectal swabs were directly inoculated onto Mueller-Hinton plates and analyzed according to both methods. RESULTS: A total of 41 (16%) and 208 (84%) were positive and negative for ESssL, respectively. Twelve (29%) and 20 (49%) of the 41 rectal swabs positive for ESssL were detected after 24h of incubation with the double-disk synergy test and the Mastdiscs method, respectively (P=0.013). One hundred fifty-eight (76%) et 183 (88%) of the 208 rectal swabs were detected negative for ESssL after 24h of incubation with the double-disk synergy test and the Mastdiscs method, respectively (P<0.001). Finally, 79 (32%) and 46 (18%) rectal swabs respectively inoculated according to the double-disk synergy test and the Mastdiscs method were inconclusive after 24h of incubation. The better performance of the Mastdiscs method was due to an easier detection of cephalosporinase producing bacteria. CONCLUSIONS: The Mastdiscs method is a simple phenotypic method that detects more easily ESssL and non-ESssL producing bacteria in rectal swab.
Mots-clé
Bacterial Proteins/*biosynthesis, beta-Lactamases/*biosynthesis, Drug Resistance, Multiple, Bacterial/genetics, Enterobacter/growth & development/isolation & purification, Enterobacteriaceae Infections/*diagnosis/microbiology, Enterobacteriaceae/*isolation & purification/metabolism, Humans, Klebsiella/growth & development/isolation & purification, Microbial Sensitivity Tests, Microbiological Techniques/instrumentation/*methods, Reagent Kits, Diagnostic, Rectum/*microbiology
Création de la notice
18/07/2019 13:48
Dernière modification de la notice
21/08/2019 6:33
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