RNAi screening reveals proteasome- and Cullin3-dependent stages in vaccinia virus infection.
Détails
ID Serval
serval:BIB_8FF9ED62B978
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
RNAi screening reveals proteasome- and Cullin3-dependent stages in vaccinia virus infection.
Périodique
Cell reports
ISSN
2211-1247 (Electronic)
Statut éditorial
Publié
Date de publication
25/10/2012
Peer-reviewed
Oui
Volume
2
Numéro
4
Pages
1036-1047
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
A two-step, automated, high-throughput RNAi silencing screen was used to identify host cell factors required during vaccinia virus infection. Validation and analysis of clustered hits revealed previously unknown processes during virus entry, including a mechanism for genome uncoating. Viral core proteins were found to be already ubiquitinated during virus assembly. After entering the cytosol of an uninfected cell, the viral DNA was released from the core through the activity of the cell's proteasomes. Next, a Cullin3-based ubiquitin ligase mediated a further round of ubiquitination and proteasome action. This was needed in order to initiate viral DNA replication. The results accentuate the value of large-scale RNAi screens in providing directions for detailed cell biological investigation of complex pathways. The list of cell functions required during poxvirus infection will, moreover, provide a resource for future virus-host cell interaction studies and for the discovery of antivirals.
Mots-clé
Computational Biology, Cullin Proteins/metabolism, DNA Replication, DNA, Viral, HeLa Cells, Humans, Proteasome Endopeptidase Complex/metabolism, RNA Interference, RNA, Small Interfering/metabolism, Ubiquitination, Vaccinia/metabolism, Vaccinia/pathology, Vaccinia/virology, Vaccinia virus/genetics, Vaccinia virus/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
09/06/2023 16:03
Dernière modification de la notice
28/07/2023 6:59