gp120 is present on the plasma membrane of apoptotic CD4 cells prepared from lymph nodes of HIV-1-infected individuals: an immunoelectron microscopic study

Détails

ID Serval
serval:BIB_8FF0FDD7D03D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
gp120 is present on the plasma membrane of apoptotic CD4 cells prepared from lymph nodes of HIV-1-infected individuals: an immunoelectron microscopic study
Périodique
AIDS
Auteur(s)
Sunila  I., Vaccarezza  M., Pantaleo  G., Fauci  A. S., Orenstein  J. M.
ISSN
0269-9370 (Print)
Statut éditorial
Publié
Date de publication
01/1997
Volume
11
Numéro
1
Pages
27-32
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan
Résumé
OBJECTIVE: To study whether free gp120 can be detected on the plasma membranes of apoptotic CD4+ T lymphocytes in lymph nodes from HIV-positive patients. METHODS: Lymph-node cell suspensions prepared from three HIV-positive patients were studied by pre-embedding, double-immunogold-labeling to identify cell type, determine cell morphology, and detect the presence of bound gp120 molecules. Cells were classified by their surface antigens as helper/inducer T lymphocytes (CD4+), cytotoxic/suppressor T lymphocytes (CD8+), B cells (CD20+), and total lymphocytes [CD45+, leukocyte common antigen (LCA)+]. RESULTS: gp120 colabelled with both apoptotic and normal CD4+ T lymphocytes and LCA+ cells, but not with either apoptotic or normal CD8+ T lymphocytes or B cells. gp120 was more often identified on apoptotic than on normal CD4+ T lymphocytes. The gp120 and CD45 label were often colocalized. HIV particles were not identified to be associated with or budding from either normal or apoptotic lymphocytes. CONCLUSIONS: Free gp120 is found bound to CD4+ T cells in lymph nodes of HIV-infected individuals and potentially mark them for premature death by apoptosis.
Mots-clé
Antigens, CD45/analysis Apoptosis CD4-Positive T-Lymphocytes/*virology Cell Membrane/virology HIV Envelope Protein gp120/*analysis HIV Infections/*immunology/virology *Hiv-1 Humans Immunophenotyping Lymph Nodes/immunology/*pathology Microscopy, Immunoelectron
Pubmed
Web of science
Création de la notice
25/01/2008 16:14
Dernière modification de la notice
20/08/2019 15:53
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