Longitudinal monitoring of endogenous steroids in human serum by UHPLC-MS/MS as a tool to detect testosterone abuse in sports.

Détails

ID Serval
serval:BIB_8FDC86128860
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Longitudinal monitoring of endogenous steroids in human serum by UHPLC-MS/MS as a tool to detect testosterone abuse in sports.
Périodique
Analytical and bioanalytical chemistry
Auteur(s)
Ponzetto F., Mehl F., Boccard J., Baume N., Rudaz S., Saugy M., Nicoli R.
ISSN
1618-2650 (Electronic)
ISSN-L
1618-2642
Statut éditorial
Publié
Date de publication
01/2016
Peer-reviewed
Oui
Volume
408
Numéro
3
Pages
705-719
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Publication types: Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't

Résumé
The detection of testosterone abuse in sports is routinely achieved through the 'steroidal module' of the Athlete Biological Passport by GC-MS(/MS) quantification of selected endogenous anabolic androgenic steroids (EAAS) from athletes' urines. To overcome some limitations of the "urinary steroid profile" such as the presence of confounding factors (ethnicity, enzyme polymorphism, bacterial contamination, and ethanol), ultrahigh performance liquid chromatography (UHPLC) measurements of blood concentrations of testosterone, its major metabolites, and precursors could represent an interesting and complementary strategy. In this work, two UHPLC-MS/MS methods were developed for the quantification of testosterone and related compounds in human serum, including major progestogens, corticoids, and estrogens. The validated methods were then used for the analyses of serum samples collected from 19 healthy male volunteers after oral and transdermal testosterone administration. Results from unsupervised multiway analysis allowed variations of target analytes to be assessed simultaneously over a 96-h time period. Except for alteration of concentration values due to the circadian rhythm, which concerns mainly corticosteroids, DHEA, and progesterone, significant variations linked to the oral and transdermal testosterone administration were observed for testosterone, DHT, and androstenedione. As a second step of analysis, the longitudinal monitoring of these biomarkers using intra-individual thresholds showed, in comparison to urine, significant improvements in the detection of testosterone administration, especially for volunteers with del/del genotype for phase II UGT2B17 enzyme, not sensitive to the main urinary marker, T/E ratio. A substantial extension of the detection window after transdermal testosterone administration was also observed in serum matrix. The longitudinal follow-up proposed in this study represents a first example of 'blood steroid profile' in doping control analysis, which can be proposed in the future as a complement to the 'urinary module' for improving steroid abuse detection capabilities.

Mots-clé
Adult, Doping in Sports, Humans, Longitudinal Studies, Male, Steroids/blood, Substance Abuse Detection/methods, Tandem Mass Spectrometry/methods, Testosterone/administration & dosage, Young Adult
Pubmed
Web of science
Création de la notice
11/04/2016 15:08
Dernière modification de la notice
20/08/2019 15:53
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