Two-dimensional electrophoresis as an aid in the analysis of the clonality of immunoglobulins

Détails

ID Serval
serval:BIB_8EDF67FC44D5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Two-dimensional electrophoresis as an aid in the analysis of the clonality of immunoglobulins
Périodique
Electrophoresis
Auteur⸱e⸱s
Tissot  J. D., Schneider  P., Hohlfeld  P., Spertini  F., Hochstrasser  D. F., Duchosal  M. A.
ISSN
0173-0835 (Print)
Statut éditorial
Publié
Date de publication
12/1993
Volume
14
Numéro
12
Pages
1366-1371
Langue
anglais
Notes
Comparative Study Journal Article Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
In this paper, we summarize our five-year observation of two-dimensional polyacrylamide gel electrophoretic (2-D PAGE) analysis of immunoglobulin (Ig) light (L) chain patterns on serum/plasma and/or purified human Ig, and compare this technique with agarose electrophoresis and/or immunofixation examination. Polyclonal Ig L chains were seen as large "fuzzy" areas with several zones of high density. The majority (71%) of the monoclonal Ig L chains of monoclonal gammopathy detected by conventional electrophoresis appeared as a single large and well-defined spot on 2-D PAGE analysis, with the remaining appearing as multiple spots. The presence of oligoclonal Ig, reflected by multiple spots in 2-D PAGE, and several bands in immunofixation, was observed in 5 of 26 patients after allogeneic bone marrow transplantation and in 5 patients with tumors. In the majority (77%) of hypergammaglobulinemia, L chains appeared as a wide spread of small and well-defined spots in 2-D PAGE analysis. This pattern suggested oligoclonal Ig-secreting B cell clone expansion, and corresponding abnormalities were not detected with immunofixation. 2-D PAGE analysis also detected oligoclonal Ig expansions whereas conventional electrophoretic examination was normal in 10 additional patients after bone marrow transplantation, and in 6 of 10 immunocompetent patients with acute severe infections. Analysis of the Ig L chain pattern of a severe combined immune-deficient mouse populated with human peripheral blood leukocytes confirmed the skewed human Ig production in the model. In summary, 2-D PAGE appears to be a sensitive tool for the analysis of Ig diversity in various clinical situations.
Mots-clé
Animals Antibodies, Monoclonal/*blood B-Lymphocytes/immunology Bone Marrow Transplantation Clone Cells/immunology Electrophoresis, Agar Gel *Electrophoresis, Gel, Two-Dimensional Humans Hypergammaglobulinemia/immunology Immunoglobulin Light Chains/*blood Infection/immunology Mice Mice, SCID Paraproteinemias/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 15:34
Dernière modification de la notice
20/08/2019 14:52
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