Two-dimensional electrophoresis as an aid in the analysis of the clonality of immunoglobulins
Détails
ID Serval
serval:BIB_8EDF67FC44D5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Two-dimensional electrophoresis as an aid in the analysis of the clonality of immunoglobulins
Périodique
Electrophoresis
ISSN
0173-0835 (Print)
Statut éditorial
Publié
Date de publication
12/1993
Volume
14
Numéro
12
Pages
1366-1371
Langue
anglais
Notes
Comparative Study Journal Article Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
In this paper, we summarize our five-year observation of two-dimensional polyacrylamide gel electrophoretic (2-D PAGE) analysis of immunoglobulin (Ig) light (L) chain patterns on serum/plasma and/or purified human Ig, and compare this technique with agarose electrophoresis and/or immunofixation examination. Polyclonal Ig L chains were seen as large "fuzzy" areas with several zones of high density. The majority (71%) of the monoclonal Ig L chains of monoclonal gammopathy detected by conventional electrophoresis appeared as a single large and well-defined spot on 2-D PAGE analysis, with the remaining appearing as multiple spots. The presence of oligoclonal Ig, reflected by multiple spots in 2-D PAGE, and several bands in immunofixation, was observed in 5 of 26 patients after allogeneic bone marrow transplantation and in 5 patients with tumors. In the majority (77%) of hypergammaglobulinemia, L chains appeared as a wide spread of small and well-defined spots in 2-D PAGE analysis. This pattern suggested oligoclonal Ig-secreting B cell clone expansion, and corresponding abnormalities were not detected with immunofixation. 2-D PAGE analysis also detected oligoclonal Ig expansions whereas conventional electrophoretic examination was normal in 10 additional patients after bone marrow transplantation, and in 6 of 10 immunocompetent patients with acute severe infections. Analysis of the Ig L chain pattern of a severe combined immune-deficient mouse populated with human peripheral blood leukocytes confirmed the skewed human Ig production in the model. In summary, 2-D PAGE appears to be a sensitive tool for the analysis of Ig diversity in various clinical situations.
Mots-clé
Animals Antibodies, Monoclonal/*blood B-Lymphocytes/immunology Bone Marrow Transplantation Clone Cells/immunology Electrophoresis, Agar Gel *Electrophoresis, Gel, Two-Dimensional Humans Hypergammaglobulinemia/immunology Immunoglobulin Light Chains/*blood Infection/immunology Mice Mice, SCID Paraproteinemias/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 16:34
Dernière modification de la notice
20/08/2019 15:52