Rapid identification of malaria vaccine candidates based on alpha-helical coiled coil protein motif.

Détails

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Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_8ED3445AE0F0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Rapid identification of malaria vaccine candidates based on alpha-helical coiled coil protein motif.
Périodique
Plos One
Auteur⸱e⸱s
Villard V., Agak G.W., Frank G., Jafarshad A., Servis C., Nébié I., Sirima S.B., Felger I., Arevalo-Herrera M., Herrera S., Heitz F., Bäcker V., Druilhe P., Kajava A.V., Corradin G.
ISSN
1932-6203[electronic], 1932-6203[linking]
Statut éditorial
Publié
Date de publication
2007
Volume
2
Numéro
7
Pages
e645
Langue
anglais
Résumé
To identify malaria antigens for vaccine development, we selected alpha-helical coiled coil domains of proteins predicted to be present in the parasite erythrocytic stage. The corresponding synthetic peptides are expected to mimic structurally "native" epitopes. Indeed the 95 chemically synthesized peptides were all specifically recognized by human immune sera, though at various prevalence. Peptide specific antibodies were obtained both by affinity-purification from malaria immune sera and by immunization of mice. These antibodies did not show significant cross reactions, i.e., they were specific for the original peptide, reacted with native parasite proteins in infected erythrocytes and several were active in inhibiting in vitro parasite growth. Circular dichroism studies indicated that the selected peptides assumed partial or high alpha-helical content. Thus, we demonstrate that the bioinformatics/chemical synthesis approach described here can lead to the rapid identification of molecules which target biologically active antibodies, thus identifying suitable vaccine candidates. This strategy can be, in principle, extended to vaccine discovery in a wide range of other pathogens.
Mots-clé
Animals, Antibodies, Protozoan/chemistry, Antibodies, Protozoan/genetics, Circular Dichroism, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Indirect/methods, Genome, Humans, Malaria Vaccines/chemistry, Malaria Vaccines/genetics, Mice, Mice, Inbred Strains, Peptide Fragments/chemistry, Peptide Fragments/immunology, Peptides/chemical synthesis, Peptides/chemistry, Plasmodium/genetics, Protein Conformation, Protozoan Proteins/chemistry, Protozoan Proteins/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:55
Dernière modification de la notice
20/08/2019 15:52
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