Tumor necrosis factor-alpha and alphaB-crystallin up-regulation during antibody-mediated demyelination in vitro: a putative protective mechanism in oligodendrocytes.

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_8EBF23262FAA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Tumor necrosis factor-alpha and alphaB-crystallin up-regulation during antibody-mediated demyelination in vitro: a putative protective mechanism in oligodendrocytes.
Périodique
Journal of Neuroscience Research
Auteur(s)
Duvanel C.B., Monnet-Tschudi F., Braissant O., Matthieu J.M., Honegger P.
ISSN
0360-4012 (Print)
ISSN-L
0360-4012
Statut éditorial
Publié
Date de publication
2004
Volume
78
Numéro
5
Pages
711-722
Langue
anglais
Résumé
By using an in vitro model of antibody-mediated demyelination, we investigated the relationship between tumor necrosis factor-alpha (TNF-alpha) and heat shock protein (HSP) induction with respect to oligodendrocyte survival. Differentiated aggregate cultures of rat telencephalon were subjected to demyelination by exposure to antibodies against myelin oligodendrocyte glycoprotein (MOG) and complement. Cultures were analyzed 48 hr after exposure. Myelin basic protein (MBP) expression was greatly decreased, but no evidence was found for either necrosis or apoptosis. TNF-alpha was significantly up-regulated. It was localized predominantly in neurons and to a lesser extent in astrocytes and oligodendrocytes, and it was not detectable in microglial cells. Among the different HSPs examined, HSP32 and alphaB-crystallin were up-regulated; they may confer protection from oxidative stress and from apoptotic death, respectively. These results suggest that TNF-alpha, often regarded as a promoter of oligodendroglial death, could alternatively mediate a protective pathway through alphaB-crystallin up-regulation.
Mots-clé
2',3'-Cyclic-Nucleotide Phosphodiesterases/metabolism, Animals, Antibodies/adverse effects, Apoptosis/physiology, Blotting, Western/methods, Cells, Cultured, Complement System Proteins/adverse effects, Crystallins/metabolism, Demyelinating Diseases/chemically induced, Demyelinating Diseases/metabolism, Disease Models, Animal, Drug Interactions, Embryo, Mammalian, Glial Fibrillary Acidic Protein/metabolism, Heme Oxygenase (Decyclizing)/metabolism, Heme Oxygenase-1, Immunohistochemistry/methods, In Situ Hybridization/methods, Myelin Basic Proteins/genetics, Myelin Basic Proteins/metabolism, Myelin Proteins, Myelin-Associated Glycoprotein/immunology, Necrosis/metabolism, Neurons/drug effects, Neurons/metabolism, Oligodendroglia/drug effects, Rats, Telencephalon/cytology, Tumor Necrosis Factor-alpha/metabolism, Up-Regulation
Pubmed
Web of science
Création de la notice
24/01/2008 14:12
Dernière modification de la notice
20/08/2019 15:52
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