Expression of common acute lymphoblastic leukemia antigen (CALLA) on human malignant melanoma cell lines.

Détails

ID Serval
serval:BIB_8E249F8BCC88
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Expression of common acute lymphoblastic leukemia antigen (CALLA) on human malignant melanoma cell lines.
Périodique
Journal of Immunology
Auteur(s)
Carrel S., Schmidt-Kessen A., Mach J.P., Heumann D., Girardet C.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Statut éditorial
Publié
Date de publication
1983
Volume
130
Numéro
5
Pages
2456-2460
Langue
anglais
Résumé
Fifteen human melanoma cells lines were tested by an antibody-binding radioimmunoassay using a monoclonal antibody (A12) directed against the common acute lymphoblastic leukemia antigen (CALLA). Cells from six melanoma lines were found to react with this antibody. The level of antigen and the percentage of positive cells in these six melanoma lines showed wide variation, as demonstrated by analysis in the fluorescence-activated cell sorter (FACS). Immunoprecipitation of solubilized 125I-labeled membrane proteins from CALLA positive melanoma cells with A12 monoclonal antibody revealed a major polypeptide chain with an apparent m.w. of 100,000 daltons, characteristic for CALLA as determined on SDS-polyacrylamide gel electrophoresis. The expression of CALLA on MP-6 melanoma cells was modulated when the cells were cultured in the presence of A12 antibody. Reexpression of CALLA on these cells occurred within 5 days after transfer of the modulated cells into medium devoid of monoclonal antibody.
Mots-clé
Animals, Antibodies, Monoclonal/analysis, Antigen-Antibody Reactions, Antigens, Neoplasm/analysis, Antigens, Neoplasm/immunology, Cell Line, Fluorescent Antibody Technique, Hematopoietic Stem Cells/analysis, Hematopoietic Stem Cells/immunology, Humans, Leukemia, Lymphoid/immunology, Melanoma/analysis, Melanoma/immunology, Mice, Mice, Inbred BALB C, Molecular Weight, Rabbits
Pubmed
Web of science
Création de la notice
25/04/2013 13:51
Dernière modification de la notice
20/08/2019 14:52
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