Role of CD8 in aberrant function of cytotoxic T lymphocytes.

Détails

Ressource 1Télécharger: BIB_8D52DCE20D43.P001.pdf (1674.61 [Ko])
Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_8D52DCE20D43
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Role of CD8 in aberrant function of cytotoxic T lymphocytes.
Périodique
The Journal of experimental medicine
Auteur⸱e⸱s
Kessler B., Hudrisier D., Cerottini J.C., Luescher I.F.
ISSN
0022-1007
Statut éditorial
Publié
Date de publication
1997
Peer-reviewed
Oui
Volume
186
Numéro
12
Pages
2033-2038
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Using H-2Kd-restricted photoprobe-specific cytotoxic T lymphocyte (CTL) clones, which permit assessment of T cell receptor (TCR)-ligand interactions by TCR photoaffinity labeling, we observed that the efficiency of antigen recognition by CTL was critically dependent on the half-life of TCR-ligand complexes. We show here that antigen recognition by CTL is essentially determined by the frequency of serial TCR engagement, except for very rapid dissociations, which resulted in aberrant TCR signaling and antagonism. Thus agonists that were efficiently recognized exhibited rapid TCR-ligand complex dissociation, and hence a high frequency of serial TCR engagement, whereas the opposite was true for weak agonists. Surprisingly, these differences were largely accounted for by the coreceptor CD8. While it was known that CD8 substantially decreases TCR-ligand complex dissociation, we observed in this study that this effect varied considerably among ligand variants, indicating that epitope modifications can alter the CD8 contribution to TCR-ligand binding, and hence the efficiency of antigen recognition by CTL.
Mots-clé
Affinity Labels, Animals, Antigen-Antibody Reactions, Antigens, CD8/immunology, Antigens, CD8/metabolism, Cytotoxicity, Immunologic, Kinetics, Ligands, Mice, Photochemistry, Protein Binding, Receptors, Antigen, T-Cell/metabolism, T-Lymphocytes, Cytotoxic/immunology, T-Lymphocytes, Cytotoxic/physiology, Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:20
Dernière modification de la notice
20/08/2019 15:51
Données d'usage