Regulated laminin-332 expression in human islets of Langerhans

Détails

ID Serval
serval:BIB_8D048E66BE49
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Regulated laminin-332 expression in human islets of Langerhans
Périodique
FASEB J
Auteur⸱e⸱s
Armanet M., Wojtusciszyn A., Morel P., Parnaud G., Rousselle P., Sinigaglia C., Berney T., Bosco D.
ISSN
1530-6860 (Electronic)
ISSN-L
0892-6638
Statut éditorial
Publié
Date de publication
12/2009
Volume
23
Numéro
12
Pages
4046-55
Langue
anglais
Notes
Armanet, Mathieu
Wojtusciszyn, Anne
Morel, Philippe
Parnaud, Geraldine
Rousselle, Patricia
Sinigaglia, Corinne
Berney, Thierry
Bosco, Domenico
eng
Research Support, Non-U.S. Gov't
FASEB J. 2009 Dec;23(12):4046-55. doi: 10.1096/fj.08-127142. Epub 2009 Aug 10.
Résumé
Laminin-332 (LN-332) is a basement membrane component known to exert a beneficial effect on rat pancreatic beta cells in vitro. In this work, we analyzed the expression of LN-332 in human islets, its expression after inflammatory insults by cytokines, and the molecular mechanisms responsible for this effect. By Western blotting and RT-PCR, we showed that LN-332 was expressed in isolated human islets. By immunofluorescence on pancreas sections, we observed that labeling was confined to endocrine cells in islets. Confocal microscopy analysis on isolated islet cells revealed that labeling was granular but did not colocalize with hormone secretory granules. LN-332 was most abundant in cultured islets compared to freshly isolated islets and was found in culture medium, which suggests that it was secreted by islets. When islets were exposed to interleukin (IL)-1beta, expression and secretion of LN-332 increased as compared to control. No effect was observed with tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma. LY294002, an inhibitor of phosphatidylinositol 3-kinase (PI3-K) activity, inhibited culture- and IL-1beta-induced LN-332 expression in islets. These results show that LN-332, known to have some beneficial effect on beta cells in vitro, is produced and secreted by endocrine islet cells and is up-regulated by stressing conditions such as culture and IL-1beta-exposure.
Mots-clé
Cell Adhesion Molecules/*genetics/*metabolism, Cells, Cultured, Cytokines/pharmacology, Gene Expression Regulation/*physiology, Glucagon/pharmacology, Humans, Insulin/pharmacology, Islets of Langerhans/drug effects/*metabolism, Phosphatidylinositol 3-Kinases/metabolism, Protein Subunits, Signal Transduction
Pubmed
Création de la notice
14/06/2021 8:58
Dernière modification de la notice
18/09/2021 5:38
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