Generation of transgene-free human induced pluripotent stem cells from erythroblasts in feeder-free conditions.

Détails

Ressource 1Télécharger: 36035798_BIB_8C8118CBC867.pdf (3768.35 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_8C8118CBC867
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Generation of transgene-free human induced pluripotent stem cells from erythroblasts in feeder-free conditions.
Périodique
STAR protocols
Auteur⸱e⸱s
Perriot S., Canales M., Mathias A., Du Pasquier R.
ISSN
2666-1667 (Electronic)
ISSN-L
2666-1667
Statut éditorial
Publié
Date de publication
16/09/2022
Peer-reviewed
Oui
Volume
3
Numéro
3
Pages
101620
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
This protocol describes the generation and characterization of human induced pluripotent stem cells (hiPSCs) from erythroblasts. A key difference with classical protocols is the reprogramming of erythroblasts from a simple blood draw as opposed to fibroblasts/keratinocytes, which requires a biopsy. Moreover, working with erythroblasts ensures that no recombination of the TCR/BCR genes occurs, as opposed to T cells and whole peripheral blood mononuclear cells-based approaches. Last, this approach uses non-integrative episomes ensuring no integration of transgenes into the hiPSCs genome. For complete details on the use and execution of this protocol, please refer to Perriot et al. (2018).
Mots-clé
Cellular Reprogramming, Erythroblasts, Humans, Induced Pluripotent Stem Cells, Leukocytes, Mononuclear, Transgenes, Biotechnology and bioengineering, Cell Biology, Cell culture, Cell isolation, Stem Cells
Pubmed
Open Access
Oui
Création de la notice
05/09/2022 8:15
Dernière modification de la notice
23/01/2024 7:30
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