ParB proteins can bypass DNA-bound roadblocks via dimer-dimer recruitment.

Détails

Ressource 1Télécharger: 35767606_BIB_8BE4A8F64584.pdf (4136.34 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_8BE4A8F64584
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
ParB proteins can bypass DNA-bound roadblocks via dimer-dimer recruitment.
Périodique
Science advances
Auteur⸱e⸱s
Tišma M., Panoukidou M., Antar H., Soh Y.M., Barth R., Pradhan B., Barth A., van der Torre J., Michieletto D., Gruber S., Dekker C.
ISSN
2375-2548 (Electronic)
ISSN-L
2375-2548
Statut éditorial
Publié
Date de publication
07/2022
Peer-reviewed
Oui
Volume
8
Numéro
26
Pages
eabn3299
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The ParABS system is essential for prokaryotic chromosome segregation. After loading at parS on the genome, ParB (partition protein B) proteins rapidly redistribute to distances of ~15 kilobases from the loading site. It has remained puzzling how this large-distance spreading can occur along DNA loaded with hundreds of proteins. Using in vitro single-molecule fluorescence imaging, we show that ParB from Bacillus subtilis can load onto DNA distantly of parS, as loaded ParB molecules themselves are found to be able to recruit additional ParB proteins from bulk. Notably, this recruitment can occur in cis but also in trans, where, at low tensions within the DNA, newly recruited ParB can bypass roadblocks as it gets loaded to spatially proximal but genomically distant DNA regions. The data are supported by molecular dynamics simulations, which show that cooperative ParB-ParB recruitment can enhance spreading. ParS-independent recruitment explains how ParB can cover substantial genomic distance during chromosome segregation, which is vital for the bacterial cell cycle.
Mots-clé
Bacillus subtilis/genetics, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Chromosome Segregation, DNA/metabolism, DNA, Bacterial/genetics, DNA, Bacterial/metabolism, Protein Binding
Pubmed
Web of science
Création de la notice
13/07/2022 11:50
Dernière modification de la notice
23/11/2022 7:13
Données d'usage