Evidence that members of the secretory aspartyl proteinase gene family, in particular SAP2, are virulence factors for Candida vaginitis

Détails

ID Serval
serval:BIB_8BBBEB080C18
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Evidence that members of the secretory aspartyl proteinase gene family, in particular SAP2, are virulence factors for Candida vaginitis
Périodique
Journal of Infectious Diseases
Auteur(s)
De Bernardis  F., Arancia  S., Morelli  L., Hube  B., Sanglard  D., Schafer  W., Cassone  A.
ISSN
0022-1899 (Print)
Statut éditorial
Publié
Date de publication
01/1999
Volume
179
Numéro
1
Pages
201-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Résumé
Virulence of Candida albicans strains with targeted disruption of secretory aspartyl proteinase genes (SAP1 to SAP6) was assessed in an estrogen-dependent rat vaginitis model. Null sap1 to sap3 but not sap4 to sap6 mutants lost most of the virulence of their parental strain SC5314. In particular, the sap2 mutant was almost avirulent in this model. Reinsertion of the SAP2 gene into this latter mutant led to the to recovery of the vaginopathic potential. The vaginal fluids of the animals infected by the wild type strain or by the sap1 or sap3 mutants expressed a pepstatin-sensitive proteinase activity in vitro. No traces of this activity were found in the vaginal fluid of rats challenged by the sap2 mutant. All strains were capable of developing true hyphae during infection. Thus, members of SAP family, in particular SAP2, play a clear pathogenic role in vaginitis and may constitute a novel target for chemoimmunotherapy of this infection.
Mots-clé
Animals Aspartic Endopeptidases/*genetics Base Sequence Candida albicans/enzymology/*genetics/*pathogenicity Candidiasis/*microbiology DNA Primers/genetics Disease Models, Animal Female *Fungal Proteins Gene Deletion *Genes, Fungal *Multigene Family Polymerase Chain Reaction Rats Rats, Wistar Vaginitis/*microbiology Virulence/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 14:40
Dernière modification de la notice
20/08/2019 14:50
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