MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia

Détails

ID Serval
serval:BIB_8BAB5C446B37
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia
Périodique
Nature
Auteur(s)
Bernhagen  J., Calandra  T., Mitchell  R. A., Martin  S. B., Tracey  K. J., Voelter  W., Manogue  K. R., Cerami  A., Bucala  R.
ISSN
0028-0836 (Print)
Statut éditorial
Publié
Date de publication
10/1993
Volume
365
Numéro
6448
Pages
756-9
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Oct 21
Résumé
Cytokines are critical in the often fatal cascade of events that cause septic shock. One regulatory system that is likely to be important in controlling inflammatory responses is the neuroendocrine axis. The pituitary, for example, is ideally situated to integrate central and peripheral stimuli, and initiates the increase in systemic glucocorticoids that accompanies host stress responses. To assess further the contribution of the pituitary to systemic inflammatory processes, we examined the secretory profile of cultured pituitary cells and whole pituitaries in vivo after stimulation with bacterial lipopolysaccharide (LPS). Here we identify macrophage migration inhibitory factor (MIF) as a major secreted protein release by anterior pituitary cells in response to LPS stimulation. Serum analysis of control, hypophysectomized and T-cell-deficient (nude) mice suggests that pituitary-derived MIF contributes to circulating MIF present in the post-acute phase of endotoxaemia. Recombinant murine MIF greatly enhances lethality when co-injected with LPS and anti-MIF antibody confers full protection against lethal endotoxaemia. We conclude that MIF plays a central role in the toxic response to endotoxaemia and possibly septic shock.
Mots-clé
Acute-Phase Reaction/blood Amino Acid Sequence Animals Antibodies/immunology/pharmacology Base Sequence Cell Line Cloning, Molecular DNA Primers Endotoxins Humans Hypophysectomy Lipopolysaccharides Macrophage Migration-Inhibitory Factors/blood/immunology/*metabolism/physiology Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Nude Molecular Sequence Data Pituitary Gland, Anterior/*metabolism Recombinant Proteins/pharmacology Sequence Homology, Amino Acid Shock, Septic/metabolism Toxemia/etiology/*metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 14:28
Dernière modification de la notice
20/08/2019 15:50
Données d'usage