Cryo-electron microscopy of vitreous sections of native biological cells and tissues

Détails

ID Serval
serval:BIB_8AE885E77F23
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cryo-electron microscopy of vitreous sections of native biological cells and tissues
Périodique
Journal of Structural Biology
Auteur(s)
Al-Amoudi  A., Norlen  L. P., Dubochet  J.
ISSN
1047-8477 (Print)
Statut éditorial
Publié
Date de publication
10/2004
Volume
148
Numéro
1
Pages
131-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
Cryo-electron microscopy of vitreous sections (CEMOVIS) is, in principle, the ultimate method of specimen preparation. It consists in ultra-rapid cooling of a sizable sample of biological material that is cut into thin sections. These are subsequently observed at low temperature in their fully hydrated vitreous state. Here, we show that CEMOVIS reveals the native state of cells and tissues with unprecedented quality and resolution. What is seen differs considerably from what conventional electron microscopy has shown previously and it is seen with more details. Our findings are demonstrated with images of cyanobacteria and skin.
Mots-clé
Animals Animals, Newborn Biopsy Cryoelectron Microscopy/*methods Cyanobacteria/*ultrastructure Epidermis/ultrastructure Freezing Humans Male Mice Microtomy/*methods Skin/*cytology/*ultrastructure Specimen Handling
Pubmed
Web of science
Création de la notice
24/01/2008 10:25
Dernière modification de la notice
20/08/2019 14:49
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