Stable growth of simian virus 40 recombinants containing multimerized enhancers.

Détails

ID Serval
serval:BIB_8A50858DE993
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Stable growth of simian virus 40 recombinants containing multimerized enhancers.
Périodique
Journal of Virology
Auteur(s)
Ondek B., Herr W.
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Statut éditorial
Publié
Date de publication
03/1991
Volume
65
Numéro
3
Pages
1596-1599
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Multiple copies of each of three genetically defined simian virus 40 protoenhancers, A, B, and C, were able to substitute for the wild-type simian virus 40 enhancer. Although the recombinant viruses grew poorly, they could be propagated without the accumulation of enhancer rearrangements that might improve viability. Mutations that inactivate the multimerized B and C protoenhancers abolished virus growth, but, unexpectedly, a mutation that inactivates the octamer-enhanson within the B protoenhancer increased virus viability. This positive effect may reflect loss of repression of the B protoenhancer by the ubiquitous octamer-motif-binding protein Oct-1.
Mots-clé
Animals, Base Sequence, Cell Line, Enhancer Elements, Genetic, Molecular Sequence Data, Recombination, Genetic, Restriction Mapping, Simian virus 40/genetics, Simian virus 40/growth & development
Pubmed
Web of science
Création de la notice
24/01/2008 15:36
Dernière modification de la notice
20/08/2019 14:49
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