C/EBPbeta couples dopamine signalling to substance P precursor gene expression in striatal neurones.

Détails

ID Serval
serval:BIB_8A3BC09D7768
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
C/EBPbeta couples dopamine signalling to substance P precursor gene expression in striatal neurones.
Périodique
Journal of neurochemistry
Auteur(s)
Kovács K.A., Steinmann M., Magistretti P.J., Halfon O., Cardinaux J.R.
ISSN
0022-3042
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
98
Numéro
5
Pages
1390-9
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Dopamine-induced changes in striatal gene expression are thought to play an important role in drug addiction and compulsive behaviour. In this study we report that dopamine induces the expression of the transcription factor CCAAT/Enhancer Binding Protein beta (C/EBP)-beta in primary cultures of striatal neurones. We identified the preprotachykinin-A (PPT-A) gene coding for substance P and neurokinin-A as a potential target gene of C/EBPbeta. We demonstrated that C/EBPbeta physically interacts with an element of the PPT-A promoter, thereby facilitating substance P precursor gene transcription. The regulation of PPT-A gene by C/EBPbeta could subserve many important physiological processes involving substance P, such as nociception, neurogenic inflammation and addiction. Given that substance P is known to increase dopamine signalling in the striatum and, in turn, dopamine increases substance P expression in medium spiny neurones, our results implicate C/EBPbeta in a positive feedback loop, changes of which might contribute to the development of drug addiction.
Mots-clé
2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine, Animals, Benzazepines, CCAAT-Enhancer-Binding Protein-beta, Cells, Cultured, Corpus Striatum, DNA Footprinting, Dopamine, Dopamine Agonists, Dopamine Antagonists, Drug Interactions, Electrophoretic Mobility Shift Assay, Embryo, Mammalian, Enzyme Inhibitors, Gene Expression, Green Fluorescent Proteins, Luciferases, Mice, Neurons, Promoter Regions, Genetic, Protein Precursors, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Tachykinins, Transfection
Pubmed
Web of science
Création de la notice
05/02/2008 13:38
Dernière modification de la notice
20/08/2019 14:49
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