The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa.

Détails

ID Serval
serval:BIB_89E875D38E32
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa.
Périodique
Molecular and General Genetics
Auteur(s)
Haas D., Holloway B.W., Schamböck A., Leisinger T.
ISSN
0026-8925 (Print)
ISSN-L
0026-8925
Statut éditorial
Publié
Date de publication
1977
Volume
154
Numéro
1
Pages
7-22
Langue
anglais
Résumé
Six loci coding for arginine biosynthetic enzymes in Pseudomonas aeruginosa strain PAO were identified by enzyme assay: argA (N-acetylglutamate synthase), argB (N-acetylglutamate 5-phosphotransferase), argC (N-acetylglutamate 5-semialdehyde dehydrogenase), argF (anabolic ornithine carbamoyl-transferase), argG (argininosuccinate synthetase), and argH (argininosuccinase). One-step mutants which had a requirement for arginine and uracil were defective in carbamoylphosphate synthase, specified by a locus designated car. To map these mutations we used the sex factor FP2 in an improved interrupted mating technique as well as the generalized transducing phages F116L and G101. We confirmed earlier studies, and found no clustering of arg and car loci. However, argA, argH, and argB were mapped on a short chromosome segment (approx. 3 min long), and argF and argG were cotransducible, but not contiguous. N-Acetylglutamate synthase, the enzyme which replenishes the cycle of acetylated intermediates in ornithine synthesis of Pseudomonas, appears to be essential for arginine synthesis since argA mutants showed no growth on unsupplemented minimal medium.
Mots-clé
Aldehyde Oxidoreductases/metabolism, Arginine/biosynthesis, Argininosuccinate Lyase/metabolism, Argininosuccinate Synthase/metabolism, Chromosome Mapping, Chromosomes, Bacterial, Conjugation, Genetic, Genes, Glutamate Synthase/metabolism, Ornithine Carbamoyltransferase/metabolism, Phosphate Acetyltransferase/metabolism, Pseudomonas aeruginosa/enzymology, Transduction, Genetic
Pubmed
Web of science
Création de la notice
25/01/2008 17:01
Dernière modification de la notice
20/08/2019 14:48
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