miR-143 interferes with ERK5 signaling, and abrogates prostate cancer progression in mice.

Détails

ID Serval
serval:BIB_89D589AB2E66
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
miR-143 interferes with ERK5 signaling, and abrogates prostate cancer progression in mice.
Périodique
PLoS One
Auteur(s)
Clapé C., Fritz V., Henriquet C., Apparailly F., Fernandez P.L., Iborra F., Avancès C., Villalba M., Culine S., Fajas L.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2009
Volume
4
Numéro
10
Pages
e7542
Langue
anglais
Résumé
BACKGROUND: Micro RNAs are small, non-coding, single-stranded RNAs that negatively regulate gene expression at the post-transcriptional level. Since miR-143 was found to be down-regulated in prostate cancer cells, we wanted to analyze its expression in human prostate cancer, and test the ability of miR-43 to arrest prostate cancer cell growth in vitro and in vivo.
RESULTS: Expression of miR-143 was analyzed in human prostate cancers by quantitative PCR, and by in situ hybridization. miR-143 was introduced in cancer cells in vivo by electroporation. Bioinformatics analysis and luciferase-based assays were used to determine miR-143 targets. We show in this study that miR-143 levels are inversely correlated with advanced stages of prostate cancer. Rescue of miR-143 expression in cancer cells results in the arrest of cell proliferation and the abrogation of tumor growth in mice. Furthermore, we show that the effects of miR-143 are mediated, at least in part by the inhibition of extracellular signal-regulated kinase-5 (ERK5) activity. We show here that ERK5 is a miR-143 target in prostate cancer.
CONCLUSIONS: miR-143 is as a new target for prostate cancer treatment.
Mots-clé
Animals, Cell Line, Tumor, Computational Biology/methods, Disease Progression, Electroporation, Humans, In Situ Hybridization, Male, Mice, Mice, Nude, MicroRNAs/metabolism, Mitogen-Activated Protein Kinase 7/metabolism, Prostatic Neoplasms/metabolism, Signal Transduction
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/03/2013 15:57
Dernière modification de la notice
20/08/2019 14:48
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