Murine Thy-1+ dendritic epidermal T cell lines express granule-associated perforin and a family of granzyme molecules

Détails

ID Serval
serval:BIB_8961C14774A0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Murine Thy-1+ dendritic epidermal T cell lines express granule-associated perforin and a family of granzyme molecules
Périodique
Immunobiology
Auteur⸱e⸱s
Krahenbuhl  O., Gattesco  S., Tschopp  J.
ISSN
0171-2985 (Print)
Statut éditorial
Publié
Date de publication
04/1992
Volume
184
Numéro
4-5
Pages
392-401
Notes
Comparative Study
Journal Article --- Old month value: Apr
Résumé
Two T cell receptor gamma/delta + murine dendritic epidermal T cell (DETC) lines with cytotoxic potential towards various tumor cell lines are shown to express perforin and granzyme A both at the mRNA and protein levels. Furthermore, mRNA transcripts for granzyme B and at least one of the other granzymes D, E, F and G are detected in amounts equivalent to a murine IL-2-dependent alpha/beta + cytotoxic T lymphocyte cell line. Hemolytic granules containing serine-esterase (granzyme A) activity are isolated from a DETC line. Thus, cytolytically-active Thy-1+ DETC lines contain the granule-associated pore-forming protein, perforin, and at least one member of each of the three subgroups of granzyme serine esterases (granzyme A, B and D/E/F/G). These data support the proposed role of gamma/delta + DETC in immune surveillance, possibly exerting cytolytic functions against virus- or parasite-infected, transformed or stressed cells.
Mots-clé
Animals Antigens, Surface/*immunology Antigens, Thy-1 Blotting, Northern Cell Line Cytoplasmic Granules/metabolism Cytotoxicity, Immunologic Dendritic Cells/*immunology Gene Expression Granzymes Membrane Glycoproteins/*immunology Membrane Proteins/*metabolism Mice Mice, Inbred Strains Pore Forming Cytotoxic Proteins RNA, Messenger/metabolism Receptors, Antigen, T-Cell, gamma-delta/immunology Serine Endopeptidases/*metabolism Skin/immunology T-Lymphocytes, Cytotoxic/*immunology Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
24/01/2008 15:19
Dernière modification de la notice
20/08/2019 14:48
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