Phage and host genetic determinants of the specific anticodon loop cleavages in bacteriophage T4-infected Escherichia coli CTr5X.

Détails

ID Serval
serval:BIB_890129A7758F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Phage and host genetic determinants of the specific anticodon loop cleavages in bacteriophage T4-infected Escherichia coli CTr5X.
Périodique
Journal of Molecular Biology
Auteur⸱e⸱s
Kaufmann G., David M., Borasio G.D., Teichmann A., Paz A., Amitsur M.
ISSN
0022-2836 (Print)
ISSN-L
0022-2836
Statut éditorial
Publié
Date de publication
1986
Volume
188
Numéro
1
Pages
15-22
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
Anticodon loop cleavages of two host tRNA species occur in bacteriophage T4-infected Escherichia coli CTr5X, a host strain restricting phage mutants deficient in polynucleotide kinase (pnk) or RNA ligase (rli). The cleavage products accumulate with the mutants but are further processed in wt infection through polynucleotide kinase and RNA ligase reactions. Inactivating mutations in stp suppress pnk- or rli- mutations in E. coli CTr5X and, as shown here, also abolish the anticodon nuclease, implicating the stp product with this activity. We show also that there exist other suppressing mutations of a pnk- (pseT2) mutation that appear not to affect the anticodon nuclease and are not in stp. It has been shown that a single locus in E. coli CTr5X, termed prr, determines the restriction of pnk- or rli- mutants. A transductant carrying prr featured upon infection the anticodon nuclease reaction products, suggesting that prr determines the specific manifestation of this activity. However, prr does not encode the tRNA species that are vulnerable to the anticodon nuclease.
Mots-clé
Anticodon/genetics, Anticodon/metabolism, Escherichia coli/genetics, Genes, Viral, Kinetics, Mutation, Nucleic Acid Hybridization, RNA, Transfer/genetics, RNA, Transfer/metabolism, RNA, Viral/genetics, Ribonucleases/metabolism, T-Phages/genetics, T-Phages/metabolism
Pubmed
Web of science
Création de la notice
13/01/2014 16:40
Dernière modification de la notice
20/08/2019 14:48
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