A putative H(+)-K(+)-ATPase is selectively expressed in surface epithelial cells of rat distal colon

Détails

ID Serval
serval:BIB_886DD75E3E03
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A putative H(+)-K(+)-ATPase is selectively expressed in surface epithelial cells of rat distal colon
Périodique
American Journal of Physiology
Auteur⸱e⸱s
Jaisser  F., Coutry  N., Farman  N., Binder  H. J., Rossier  B. C.
ISSN
0363-6143
Statut éditorial
Publié
Date de publication
10/1993
Volume
265
Numéro
4 Pt 1
Pages
C1080-9
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Oct
Résumé
Recently, a putative distal colon H(+)-K(+)-ATPase alpha-subunit has been identified and characterized (M. S. Crowson and G. E. Shull. J. Biol. Chem. 267:13740-13748, 1992). In the present study, we report the tissue and cell expression of this putative H(+)-K(+)-ATPase. The results indicate that, first, in the gut, the putative H(+)-K(+)-ATPase alpha-subunit is restricted to the distal part of the colon and is predominantly expressed in surface epithelial cells, in marked contrast to the alpha 1-subunit of Na(+)-K(+)-ATPase that is also expressed in glands. These data suggest that the H(+)-K(+)-ATPase alpha-subunit is a potential marker for terminal differentiation of distal colon. Second, in the uterus, the putative H(+)-K(+)-ATPase is restricted to the region of the myometrium between the inner and midmuscular zone that is very rich in vascular supply and nerve cells. This striking expression suggests that the H(+)-K(+)-ATPase may not be involved in the control of pH and potassium concentration of the uterine fluid but rather in distinct functions of vascular and/or nerve cells. Third, with the use of three independent and different approaches (Northern blot analysis, ribonuclease protection assay, and in situ hybridization), we were unable to detect any significant amount of H(+)-K(+)-ATPase transcripts in kidney tissue. Our data suggest that the putative distal colon H(+)-K(+)-ATPase is probably distinct from the kidney isoform. Finally, we report the sequence of a set of degenerate oligonucleotides that are useful to clone alpha-subunits of the Na(+)-K(+)-/H(+)-K(+)-ATPase gene family in different tissues and different species.
Mots-clé
Animals Base Sequence Colon/cytology/*enzymology H(+)-K(+)-Exchanging ATPase/*metabolism In Situ Hybridization Intestinal Mucosa/cytology/*enzymology Isoenzymes/metabolism Molecular Sequence Data Oligonucleotide Probes/genetics Polymerase Chain Reaction Rats Tissue Distribution
Pubmed
Création de la notice
24/01/2008 13:00
Dernière modification de la notice
20/08/2019 14:47
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