In vivo imaging of T cell delivery to tumors after adoptive transfer therapy.

Détails

ID Serval
serval:BIB_885356D459FF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vivo imaging of T cell delivery to tumors after adoptive transfer therapy.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Pittet M.J., Grimm J., Berger C.R., Tamura T., Wojtkiewicz G., Nahrendorf M., Romero P., Swirski F.K., Weissleder R.
ISSN
0027-8424
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
104
Numéro
30
Pages
12457-12461
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Adoptive transfer therapy of in vitro-expanded tumor-specific cytolytic T lymphocytes (CTLs) can mediate objective cancer regression in patients. Yet, technical limitations hamper precise monitoring of posttherapy T cell responses. Here we show in a mouse model that fused single photon emission computed tomography and x-ray computed tomography allows quantitative whole-body imaging of (111)In-oxine-labeled CTLs at tumor sites. Assessment of CTL localization is rapid, noninvasive, three-dimensional, and can be repeated for longitudinal analyses. We compared the effects of lymphodepletion before adoptive transfer on CTL recruitment and report that combined treatment increased intratumoral delivery of CTLs and improved antitumor efficacy. Because (111)In-oxine is a Food and Drug Administration-approved clinical agent, and human SPECT-CT systems are available, this approach should be clinically translatable, insofar as it may assess the efficacy of immunization procedures in individual patients and lead to development of more effective therapies.
Mots-clé
Adoptive Transfer, Animals, Antigens/immunology, Cell Line, Tumor, Cell Movement, Immunotherapy, Adoptive, Lymphopenia/immunology, Lymphopenia/pathology, Mice, Mice, Inbred BALB C, Neoplasms/immunology, Neoplasms/pathology, T-Lymphocytes/cytology, T-Lymphocytes/immunology, Tomography Scanners, X-Ray Computed
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:27
Dernière modification de la notice
20/08/2019 15:47
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