Natural 30 base pair and 69 base pair deletion variants of the LMP1 oncogene do stimulate NF-kappaB-mediated transcription

Détails

ID Serval
serval:BIB_8651B79C0021
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Natural 30 base pair and 69 base pair deletion variants of the LMP1 oncogene do stimulate NF-kappaB-mediated transcription
Périodique
Oncogene
Auteur(s)
Rothenberger  S., Bachmann  E., Berger  C., McQuain  C., Odermatt  B. F., Knecht  H.
ISSN
0950-9232 (Print)
Statut éditorial
Publié
Date de publication
05/1997
Volume
14
Numéro
17
Pages
2123-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 1
Résumé
An increasing number of reports shows a link between the Epstein-Barr virus (EBV) and lymphoid neoplasia. The latent membrane protein 1 (LMP1) is likely to play a determinant role in this process since this EBV encoded protein has oncogenic properties and is usually expressed in EBV-associated lymphoproliferative diseases (LPD), except Burkitt's lymphoma. We previously identified in LPD patients mutational hot spots and a 30 bp or 69 bp deletion in the LMP1 gene region coding for the C-terminal domain. These deletions are located in an area shown to be important for the activation of the transcription factor NF-kappaB. These findings lead us to test whether these natural deletion variants may have a functional effect. We measured the stimulation of their activity using a luciferase reporter plasmid containing NF-kappaB responsive elements. We tested the NF-kappaB inducing activity of four naturally occurring LMP1 deletion variants. Our results show that these deletion variants activate NF-kappaB to the same level as the wild-type form, indicating that the crucial residues for NF-kappaB activation are conserved among the variants isolated and lie within the last 32 amino acids of the C-terminal domain of the LMP1 oncogene.
Mots-clé
Base Composition Cells, Cultured *Gene Expression Regulation, Viral Genes, Reporter Genes, Viral/*genetics Herpesvirus 4, Human/*genetics/physiology Humans Luciferases/biosynthesis/genetics Lymphoproliferative Disorders/genetics/pathology/virology NF-kappa B/*metabolism Oncogenes/*genetics Recombinant Fusion Proteins/biosynthesis/genetics *Sequence Deletion *Transcription, Genetic Variation (Genetics) Viral Matrix Proteins/*genetics/physiology Viral Structural Proteins/*genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 14:36
Dernière modification de la notice
20/08/2019 14:45
Données d'usage