The hu-PBL-SCID mouse model. Long-term human serologic evolution associated with the xenogeneic transfer of human peripheral blood leukocytes into SCID mice

Détails

ID Serval
serval:BIB_85C5415E80AC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The hu-PBL-SCID mouse model. Long-term human serologic evolution associated with the xenogeneic transfer of human peripheral blood leukocytes into SCID mice
Périodique
Cellular Immunology
Auteur⸱e⸱s
Duchosal  M. A., Eming  S. A., McConahey  P. J., Dixon  F. J.
ISSN
0008-8749 (Print)
Statut éditorial
Publié
Date de publication
02/1992
Volume
139
Numéro
2
Pages
468-477
Langue
anglais
Notes
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb
Résumé
We present a 2-year serologic analysis of severe combined immune deficiency (SCID) mice populated with human peripheral blood leukocytes (PBL, hu-PBL-SCID mice). After 10-20 x 10(6) PBL transfer, human IgG serum levels generally increased in the SCID mouse recipient for 2 months, and thereafter decreased without returning to zero for at least 2 years. Great variability existed between different hu-PBL-SCID mice with regard to Ig serum levels even when derived from the same donor's PBL aliquot. The ratio of IgM to IgG serum levels was lower in hu-PBL-SCID mice than in the donors. The half-life of human IgG in the SCID mouse is shorter than in the human (8 days vs 23 days), suggesting a much higher production of IgG than expected from serum levels. The majority of hu-PBL-SCID mouse sera analyzed by high resolution electrophoresis had a smear appearance suggestive of diverse human Ig, generally with superimposed multiple faint mIg. Few mice developed strong human mIg, associated with lymphoproliferative diseases. In the hu-PBL-SCID mouse model, the transfer of cells from donors making antibody with defined specificity against TT and nuclear antigen resulted in the appearance of these antibodies in only a minority of the recipients.
Mots-clé
Animals Antibodies, Antinuclear/analysis Antibodies, Bacterial/analysis Half-Life Humans Immunoglobulin G/analysis Immunoglobulin M/analysis Leukocyte Transfusion Leukocytes/*immunology Mice Mice, SCID/*immunology Models, Biological Tetanus Toxoid/immunology Time Factors Transplantation, Heterologous/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 15:24
Dernière modification de la notice
20/08/2019 14:45
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