Stimulation of chick neuroblast proliferation in culture by brain extracts.
Détails
ID Serval
serval:BIB_852FD558A3B9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Stimulation of chick neuroblast proliferation in culture by brain extracts.
Périodique
Journal of neuroscience research
ISSN
0360-4012
Statut éditorial
Publié
Date de publication
1982
Peer-reviewed
Oui
Volume
8
Numéro
2-3
Pages
303-314
Langue
anglais
Résumé
Chick neuroblasts from 6-day-old embryos were grown on a collagen substrate in minimum Eagle's medium supplemented with 5% horse serum. We found that [3H]thymidine incorporation in these cultures decreased drastically from day 2 to day 3 after seeding. Addition of brain extract from 8-day-old chick embryos (CBe8) to the nutrient medium after 24 hours of culture elicited a very important stimulation of [3H]thymidine incorporation. Extracts of brains from adult animals were much less effective. It was shown that the increase of tritiated-thymidine incorporation reflected proliferation of neuroblasts in the culture, at least between day 2 and 4. The activity of the brain extracts was destroyed by trypsin treatment, which suggests that the factor responsible for the stimulation of the proliferation is a polypeptide. The CBe8 and the brain extract of adult chicken (CBa) were fractionated by gel filtration. An active fraction was eluted at a volume indicating an apparent molecular weight of roughly 70,000. At that stage of the study results suggest that a polypeptide (tentatively called neuroblast proliferation factor, NPF) present in the embryonic chick brain extract, is a proliferation factor for chick neuroblasts in primary culture.
Mots-clé
Animals, Brain, Brain Chemistry, Cell Differentiation, Cells, Cultured, Chick Embryo, Chickens, Nerve Growth Factors, Peptides, Thymidine, Tissue Extracts
Pubmed
Web of science
Création de la notice
30/03/2009 10:34
Dernière modification de la notice
20/08/2019 15:44