A liver protein fraction regulating hormone-dependent in vitro transcription from the vitellogenin genes induces their expression in Xenopus oocytes.

Détails

ID Serval
serval:BIB_8463EAB528E0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A liver protein fraction regulating hormone-dependent in vitro transcription from the vitellogenin genes induces their expression in Xenopus oocytes.
Périodique
Molecular Endocrinology
Auteur(s)
Corthésy B., Corthésy-Theulaz I., Cardinaux J.R., Wahli W.
ISSN
0888-8809[print], 0888-8809[linking]
Statut éditorial
Publié
Date de publication
02/1991
Peer-reviewed
Oui
Volume
5
Numéro
2
Pages
159-169
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Xenopus laevis oocytes were used to assay for trans-acting factors shown previously to be involved in the liver-specific regulation of the vitellogenin genes in vitro. To this end, crude liver nuclear extracts obtained from adult estrogen-induced Xenopus females were fractionated by heparin-Sepharose chromatography using successive elutions with 0.1, 0.35, 0.6, and 1.0 M KCl. When these four fractions were injected into oocytes, only the 0.6-M KCl protein fraction significantly stimulated mRNA synthesis from the endogenous B class vitellogenin genes. This same fraction induced estrogen-dependent in vitro transcription from the vitellogenin B1 promoter, suggesting that it contains at least a minimal set of basal transcription factors as well as two positive factors essential for vitellogenin in vitro transcription, i.e. the NF-I-like liver factor B and the estrogen receptor (ER). The presence of these two latter factors was determined by footprinting and gel retardation assays, respectively. In contrast, injection of an expression vector carrying the sequence encoding the ER was unable to activate transcription from the oocyte chromosomal vitellogenin genes. This suggests that the ER alone cannot overcome tissue-specific barriers and that one or several additional liver components participate in mediating tissue-specific expression of the vitellogenin genes. In this respect, we present evidence that the oocyte germinal vesicles contain an NF-I-like activity different from that found in hepatocytes of adult frogs. This observation might explain the lack of vitellogenin gene activation in oocytes injected with the ER cDNA only.
Mots-clé
Animals, Base Sequence, Blotting, Western, Female, Gene Expression Regulation/drug effects, Liver/chemistry, Male, Molecular Sequence Data, Nuclear Proteins/isolation & purification, Nuclear Proteins/pharmacology, Oocytes/metabolism, Promoter Regions, Genetic, RNA/genetics, Receptors, Estrogen/biosynthesis, Receptors, Estrogen/genetics, Transcription, Genetic/drug effects, Transcriptional Activation, Vitellogenins/genetics, Xenopus laevis
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/02/2008 11:32
Dernière modification de la notice
20/08/2019 15:44
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