Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging.

Détails

ID Serval
serval:BIB_83CD9C218AAE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging.
Périodique
FEBS Letters
Auteur(s)
Broillet M., Randin O., Chatton J.
ISSN
0014-5793
Statut éditorial
Publié
Date de publication
03/2001
Peer-reviewed
Oui
Volume
491
Numéro
3
Pages
227-232
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
The fluorescent indicator of nitric oxide (NO), 4,5-diaminofluorescein (DAF-2), and its membrane-permeable derivative (DAF-2 diacetate) have been recently developed to perform real-time biological imaging of NO. In this study, we show that DAF-2 is strongly influenced by factors other than the concentration of NO itself. Using measurements with a fluorimeter as well as fluorescence microscopy, we found that the divalent cation concentration in the medium, as well as the incident light, strongly affects the ability of DAF-2 to detect NO. Calcium, in particular, enhanced the signal detection of NO released by NO donors by up to 200 times. With multiple and longer exposures to light, no bleaching of the dye was observed but, instead, a potentiation of the fluorescence response could be measured. While these two properties will affect the use and interpretation of the hitherto acquired data with this fluorescent compound, they may also open up new possibilities for its application.
Mots-clé
Artifacts, Calcium/metabolism, Calcium/pharmacology, Cations, Divalent/pharmacology, Cell Line, Dose-Response Relationship, Drug, Dose-Response Relationship, Radiation, Fluorescein/chemistry, Fluorescein/metabolism, Fluorescence, Fluorometry/methods, Humans, Intracellular Fluid/drug effects, Intracellular Fluid/metabolism, Isotonic Solutions/metabolism, Kidney/cytology, Kidney/drug effects, Light, Nitric Oxide/analysis, Nitric Oxide/metabolism, Nitric Oxide Donors/pharmacology, Photochemistry/methods, Reproducibility of Results, Sensitivity and Specificity
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 12:01
Dernière modification de la notice
20/08/2019 15:43
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